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Adipogenesis : methods and protocols / edited by Michael S. Pepper and Melvin A. Ambele.
- Format:
- Book
- Series:
- Methods in molecular biology (Clifton, N.J.) 1940-6029
- Methods in molecular biology, 1940-6029
- Language:
- English
- Subjects (All):
- Adipose tissues--Laboratory manuals.
- Adipose tissues.
- Adipose tissues--Genetics--Laboratory manuals.
- Stem cells--Laboratory manuals.
- Stem cells.
- Molecular biology--Laboratory manuals.
- Molecular biology.
- Adipose tissues--Research.
- Genre:
- Laboratory manuals
- Physical Description:
- 1 online resource (x, 189 pages) : illustrations (some color).
- Place of Publication:
- New York, NY : Humana Press, [2025]
- Summary:
- This volume details protocols in Adipogensis research, such as stem cell line isolation, gene expression, obesity research, and drug development. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Adipogenesis: Methods and Protocols aims to be comprehensive guide for researchers in the field.
- Contents:
- Adipogenic differentiation from human embryonic stem cells through formation of embryoid bodies / Miguel Angel Olmedo-Sua´rez, Edgar Sa´nchez-Rami´rez, and Lorena Aguilar-Arnal
- Quantification of adipogenesis using flow cytometry / Carina C. Babbo, Chrisna Durandt, Melvin A. Ambele, and Michael S. Pepper
- Isolation of Wharton’s jelly-derived mesenchymal stromal/stem cells / Carina C. Babbo, Chrisna Durandt, Melvin A. Ambele, and Michael S. Pepper
- Adipogenic differentiation assay for the study of insulin signaling / Jonathan M. Gamwell, Emily Cresswell, and Katherine E. Pinnick
- Quantification of lipid droplets in adipocytes / Reabetswe R. Pitere, Carina C. Babbo, Rachel Giles, Melvin A. Ambele, and Michael S. Pepper
- RT-qPCR quantification of adipogenic marker genes (PPAR?, C/EBPa, FABP4, and CD36) / Carina C. Babbo, Reabetswe R. Pitere, Rachel Giles, Melvin A. Ambele, and Michael S. Pepper
- Adipogenic differentiation of human adipose-derived mesenchymal stromal/stem cells (ASCs) / Ladislaus Tapiwa Adam, Reabetswe R. Pitere, Melvin A. Ambele, and Michael S. Pepper
- Western blot detection of adipogenic protein / Pontsho Moela
- Measurement of intracellular reactive oxygen species during adipogenesis / Ladislaus Tapiwa Adam, Melvin A. Ambele, and Michael S. Pepper
- Oxygen consumption and extracellular acidification rate in adipogenic differentiated human mesenchymal stromal/stem cells / Miguel Angel Olmedo-Sua´rez, Edgar Sa´nchez-Rami´rez, Ximena del Toro-Rios, Lilia G. Noriega, and Lorena Aguilar-Arnal
- In vitro expansion of human adipose-derived stem cells (ASCs) / Ladislaus Tapiwa Adam, Melvin A. Ambele, and Michael S. Pepper
- siRNA silencing of adipogenic gene in ASCs / Pontsho Moela
- Freezing and thawing of adipose-derived stromal/stem cells (ASCs) / Ladislaus Tapiwa Adam, Michael S. Pepper, and Melvin A. Ambele
- Isolation and characterization of mouse adipose-derived stromal/stem cells / Kimberly Thando Peta, Michael S. Pepper, and Melvin A. Ambele
- Isolation of adipose-derived mesenchymal stromal/stem cells / Anastasios Athanasiadis, Melvin A. Ambele, and Michael S. Pepper
- Isolation and purification of adipocyte-derived extracellular vesicles (AdEVs) using size-exclusion chromatography / Thabiso Eric Motaung
- Differentiation of adipose-derived stromal/stem cells into osteocytes / Jamie de Beer and Michael S. Pepper
- Differentiation of mesenchymal stromal/stem cells from induced pluripotent stem cells / Alex Leah Marais, Murray Glen Logan, Juanita Mellet, Melvin A. Ambele, and Michael S. Pepper
- Isolation and characterization of equine mesenchymal stromal/stem cells / Lisa Marie Bosman, Melvin A. Ambele, and Michael S. Pepper.
- Notes:
- Includes bibliographical references and index.
- Online resource; title from PDF title page (Springer Link Nature, viewed March 1, 2026).
- Other Format:
- Print version: Adipogenesis
- ISBN:
- 9781071646076
- 1071646079
- OCLC:
- 1522115856
- Publisher Number:
- CIPO000228368
- CIPO000228367
- Access Restriction:
- Restricted for use by site license
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