Zymography : principles, process, and pitfalls. Volume 1 / edited by Raouf A. Khalil.

Format:

Book

Contributor:

Khalil, Raouf A., editor.

Series:

Methods in molecular biology (Clifton, N.J.)

Methods in molecular biology, 1940-6029

Language:

English

Subjects (All):

Enzymes--Analysis--Laboratory manuals.

Enzymes.

Enzyme activation--Laboratory manuals.

Enzyme activation.

Enzymology.

Physical Description:

1 online resource (xv, 270 pages) : illustrations.

Place of Publication:

New York, NY : Humana Press, 2025.

Summary:

"Volume 1 in this book describes how to determine the activity of different isozymes, allozymes, and families of proteinases to advance the fields of enzymology and molecular evolution, and provides useful biomarkers for various biological processes, pathological conditions, and clinical disorders. The chapters in Volume 1 are organized in four parts. Part I introduces gelatin zymography principle, method and precautions, and a practical view of zymography. Part II provides details on the use of zymography to assess various endopeptidases, and includes updates on protease zymography, protocols for gelatin zymography, detection of gelatinases by substrate zymography, unveiling matrix metalloproteinase (MMP) dynamics, and measuring MMPs activity by gelatin zymography. Part III includes chapters on one- and two-dimensional zymography for assessing proteases, loading-controlled zymography, and the use of gel diffusion-based gelatin zymography to analyze MMP-2 and MMP-9 activity in cell culture. Part IV focuses on the use of zymography to assess protease inhibitors and includes chapters that describe polyacrylamide gel electrophoresis as a tool to identify potential inhibitors of protein glycation and analyze glycation-induced protein cross-linking inhibition, kinetics of protease thermal inactivation, and reversible inhibition of cysteine proteases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls"-- Provided by publisher.

Contents:

Gelatin zymography : Principle, method and application in cancer research / Luomeng Qian, Sihe Zhang

Recent updates on protease zymography / Abinaya Kanagaraja, Raman Pachaiappan

Gelatin zymography : A simple and effective technique to detect gelatinases / Meilang Xue, Haiyan Lin, Christer J. Jackson, Lyn March

Detection of gelatinases by substrate zymography / Emanuela Falcinelli, Paolo Gresele

Zymography : Unveiling matrix metalloproteinase dynamics / Qinghe Meng, Adam Novak, Erin Rayhill

Measuring matrix metalloproteinases activity by gelatin zymography / Kelli C. Micocci, Uliana S. Stotzer, Milene N.O. Moritz

A practical view of in-gel gelatin zymography of matrix metalloproteinases 2 and 9 in cardiac tissue / Anderson Seiji Soares Fujimori, Bertha Furlan Polegato

Gel zymography for the evaluation of matrix metalloproteinase-2 and -9 in aorta and kidney tissues / Grazieli Beatriz De Oliveira Gonçalves, Vanessa De Almeida Belo, Carla Speroni Ceron

Analysis of enzymatic activity of matrix metalloproteinases by collagen zymography utilizing extracted collagen from bovine muscle tissue / Larissa A. Koulicoff, Michael D. Chao

Electrophoretic zymography of protease using azocasein / Jônatas Carvalho-Silva, Ligia M. G. Fernandes, Attilio Converti, Tatiana S. Porto

Advanced zymographic techniques for detection and in-depth characterization of microbial proteases in enzymology research / Sondes Mechri, Alexandre Noiriel, Abdelkarim Abousalham, Bassem Jaouadi

In-gel activity assay for glycosidases / Nina V. Stourman

Uncovering hyaluronidase activity using hyaluronan zymography in biological samples / Kristina Nešporová, Kateřina Lehká, Pavlína Musilová

Leveraging zymography to discover and investigate nuclear proteases / Benjamin H. Weekley, Olivia G. Henshaw, Judd C. Rice

An overview of chitinase zymography : Past and present methods and protocols / Pinki Kumari Singh, Raman Pachaiappan

One- and two-dimensional zymography methods for assessing the family of proteases / Sohail S. Qazi

Loading-controlled zymography / Aleksandra Franczak, Paul Jurasz

Gel diffusion-based gelatin zymography to analyze MMP-2 and MMP-9 activity in cell culture / Priyadarshini Gupta, Vibha Rani

Analysis of protein glycation inhibition using polyacrylamide gel electrophoresis / Handunge Kumudu Irani Perera

Analysis of glycation-induced protein cross-linking inhibition using SDS-polyacrylamide gel electrophoresis / Handunge Kumudu Irani Perera

Kinetics of protease thermal inactivation / Natalija Andrejević, Natalija Polović, Jelica Milošević

Reversible inhibition of cysteine proteases using S-methyl methanethiosulfonate / Srđan Marković, Natalija Polović.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed May 14, 2025).

Other Format:

Print version: Zymography.

ISBN:

9781071644782

1071644785

OCLC:

1519552261

Publisher Number:

CIPO000208851

CIPO000208850

Access Restriction:

Restricted for use by site license