The surfaceome : methods and protocols / edited by Csaba Matta, Ali Mobasheri, Roland Takács.

Format:

Book

Contributor:

Matta, Csaba, editor.

Mobasheri, Ali, editor.

Takács, Roland, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 1940-6029 2908.

Methods in molecular biology, 1940-6029 ; 2908

Language:

English

Subjects (All):

Cell membranes--Research--Methodology.

Cell membranes.

Physical Description:

1 online resource (xiii, 279 pages) : illustrations (chiefly color).

Place of Publication:

New York, NY : Humana Press, [2025]

Summary:

This volume explores a collection of methodologies used to study the complexities of biological membranes and the associated surfaceome. The chapters in this book aim to facilitate the adoption of innovative methods to help researchers advance their understanding of the biology of membranes and their role in health and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble shooting and avoiding known pitfalls. Comprehensive and cutting-edge, The Surfaceome: Methods and Protocols is a valuable resource for both novice and expert researchers looking to learn more about this important and developing field"-- Provided by publisher.

Contents:

Introduction : Methods for studying the plasma membrane and the surfaceome / Roland Takács, Ali Mobasheri, and Csaba Matta

Optimized biotinylated peptide detection method for characterizing the cell surface proteome / Tamás Langó, Katalin Kuffa, and Gábor E. Tusnády

Proximity labeling of cell surface proteins via cell surface remodeling / Zak Vilen, Jia Meng Pang, and Mia L. Huang

BioID-based proximity mapping of transmembrane proteins in human airway cell models / Melissa Iazzi, Audrey Astori, Jonathan St-Germain, Sara Sadeghi, Brian Raught, and Gagan D. Gupta

Aminooxy biotin-based characterization of the surfaceome of chondrogenic cells / Patrik Kovács, David J. Boocock, Clare Coveney, Ali Mobasheri, and Csaba Matta

Isolation of the surfaceome from primary human epidermal melanocytes using glycocapture and shotgun proteomics / Tibor Hajdu, Minh Ngoc Nguyen, Emese Zsigrai, Clare Coveney, David J. Boocock, and Csaba Matta

Dissecting the heterogeneity of senescent cells through CITE-seq integration / Kotb Abdelmohsen, Krystyna Mazan-Mamczarz, Dimitrios Tsitsipatis, Martina Rossi, Rachel B. Munk, and Myriam Gorospe

Metal organic layers–based sample preparation method for membrane proteome analysis / Jiang Qianqian, Jiang Bo, Zhang Yukui, and Zhang Lihua

Updated methods for elucidating bacterial surfaceomes / Veronica M. Jarocki, Maria C. Rezcallah, Pauline M. L. Coulon, Matthew P. Padula, and Steven P. Djordjevic

Functional characterization of ion channels in planar lipid bilayers / Andras Szollosi

Direct assessment of temperature sensitivity of the TRPM2 channel with the patch clamp technique / Adam V. Toth and Adam Bartok

Measuring molecular interactions with subcellular resolution : Single-cell FRET using the quantitative three-filterset (intensity-based) approach / István Rebenku, Cameron B. Lloyd, and György Vereb

Quantifying the electrophysiological properties of cells using dielectrophoresis / Krista S. P. Clarke, Oreoluwa V. Griffiths, Kai Hoettges, Rebecca Lewis, and Fatima H. Labeed

Biophysical profiling of protein corona on red blood cell-derived extracellular vesicles (REVs) : Linear dichroism and microfluidic resistive pulse sensing separate surface clearing from vesicle disruption / Tasvilla Sonallya, Anikó Gaál, Imola Cs. Szigyártó, Zoltán Varga, and Tamás Beke-Somfai

ATR-infrared spectroscopy and acoustic sensing in characterization of blood and pancreatic ductal adenocarcinoma-derived extracellular vesicles / Imola Cs. Szigyártó, Priyanka Singh, Tasvilla Sonallya, Loránd Románszki, Judith Mihály, Zoltán Wiener, Tímea Bebesi, Gábor Mészáros, Zsófia Keresztes, Michael Thompson, Zoltán Varga, and Tamás Beke-Somfai

Floating films of biological importance at the liquid–gas interface : Langmuir monolayer approach / Anita Wnętrzak, Anna Chachaj-Brekiesz, and Patrycja Dynarowicz-Latka

Protein analysis by FT-IR/ATR spectroscopy / Gabriela Graziani, Giovanni D’Atri, Elena Gabusi, and Gina Lisignoli.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed May 9, 2025).

Other Format:

Print version: Surfaceome

ISBN:

9781071644348

1071644343

OCLC:

1518947188

Access Restriction:

Restricted for use by site license