Topoisomerases : methods and protocols / edited by Joseph Deweese.

Format:

Book

Contributor:

Deweese, Joseph E. (Joseph Edward), editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 1940-6029 2928.

Methods in molecular biology, 1940-6029

Language:

English

Subjects (All):

DNA topoisomerases--Laboratory manuals.

DNA topoisomerases.

Genre:

Laboratory manuals.

Physical Description:

1 online resource (x, 234 pages) : illustrations (some color).

Place of Publication:

New York, NY : Humana Press, [2025]

Summary:

"This volume is intended to provide both fundamental methods for long-standing assays as well as recent advances in the field toward newer pathways. Chapters guide readers through methods tailored to studying prokaryotic topoisomerases including fundamental protocols for purification and basic characterization of prokaryotic enzymes, enzymology examining DNA cleavage, relaxation, decatenation, and binding to more complex assays dissecting cellular function and interactions, and bioinformatics protocol for examining protein sequence interdependencies with application across many different proteins beyond topoisomerases. Authoritative and cutting-edge, Topoisomerases: Methods and Protocols as a resource both to new investigators and to long-standing researchers in the field of topoisomerases"-- Provided by publisher.

Contents:

Expression and one-step purification of his-tagged bacterial topoisomerase subunits from Escherichia coli / Katie J. Aldred

Bacterial RADAR assay : Isolation and detection of intracellular covalent complexes / Katie J. Aldred

Bacterial topoisomerase I growth complementation assay / Thirunavukkarasu Annamalai, Yuk-Ching Tse-Dinh

DNA supercoiling catalyzed by bacterial gyrase / Samika Joshi, Neil Osheroff

DNA decatenation catalyzed by bacterial topoisomerase IV / Jillian F. Armenia, Neil Osheroff

DNA cleavage mediated by bacterial type II topoisomerases / Chelsea A. Mann, Neil Osheroff

Expression, purification, and measurement of DNA supercoil relaxation activity by eukaryotic topoisomerase II / Clark Fritsch, James M. Berger

Plasmid DNA cleavage assay with eukaryotic topoisomerase II / Allison J. Thomas, Brooke D. Latham, Addison K. O'Brian, Mattalyn R. Hardin, Joseph Deweese

Topoisomerase II DNA cleavage assay using fluorescently labelled double-stranded oligonucleotides / Ian G. Cowell, Elise M. Ling, Caroline A. Austin

Plasmid DNA binding electrophoretic mobility shift assay with eukaryotic topoisomerase II / Jeong Won Chang, Daniel Ngabonziza, Joseph Deweese

Topoisomerase II N-terminal ATPase clamp stabilization / Lauren A. Fielding, Joseph Deweese

Yeast tools for studying type II topoisomerases in budding yeast / Maureen Nie McCoy, Myna Adhikari, Karin C. Nitiss, John L. Nitiss

Use of Xenopus egg extracts to study the effects of topoisomerase poisons during vertebrate DNA replication / Sabrina X. Van Ravenstein, James M. Dewar

Inducing and monitoring liquid-liquid phase separation by type II topoisomerases / Rosa Meyo Jimenez, Joshua Jeong, James M. Berger

Analyzing topoisomerase II cleavage complexes using flow cytometry / Maria Camila Gosso, Néstor R. Aznar, Marcela B. González-Cid, Marcelo De Campos Nebel

Kinetoplast DNA decatenation assay with eukaryotic topoisomerase II / Addison K. O'Brian, Mattalyn R. Hardin, Allison J. Thomas, Brooke D. Latham, Joseph Deweese

Recombinant topoisomerase 2 production using cultured human cell lines / Anh T. Q. Cong, Matthew J. Schellenberg

Using PSICalc to identify protein sequence interdependencies / Clark E. Endsley, Matthew J. Kuhl, Thomas D. Townsley, Kirk K. Durston, Marc Soda, T. L. Wallace, Joseph Deweese.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed May 21, 2025).

Other Format:

Print version: Topoisomerases

ISBN:

9781071645505

1071645501

Publisher Number:

CIPO000228359

Access Restriction:

Restricted for use by site license