Zygotic genome activation : methods and protocols / edited by Matthew C. Good.

Format:

Book

Contributor:

Good, Matthew C., editor.

Series:

Methods in molecular biology (Clifton, N.J.) 1940-6029

Methods in molecular biology, 1940-6029

Language:

English

Subjects (All):

Embryology--Laboratory manuals.

Embryology.

Transcriptional activation--Laboratory manuals.

Transcriptional activation.

Genomes.

Physical Description:

1 online resource (xi, 264 pages) : illustrations (some color).

Place of Publication:

New York, NY : Humana Press, [2025]

Summary:

"This volume details cutting-edge techniques used to gain new and deeper insights on the fundamental regulation of zygotic genome activation (ZGA). Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Zygotic Genome Activation: Methods and Protocols aims to serve as a practical reference for researchers interested in early development and genome activation"-- Provided by publisher.

Contents:

Probing the genomic distribution of histone modifications in egg extract-treated sperm nuclei / Florian Berger, Jérôme Jullien

Canonical and variant nucleosome reprogramming from sperm to blastula / Fanju W. Meng, Patrick J. Murphy

Chromatin analyses in mouse oocytes and preimplantation embryos / Zhiyuan Chen, Chunxia Zhang, Yi Zhang

Hi-C protocol for studying chromatin spatial organization in early zebrafish development / Sergey V. Ulianov, Mariia Bazarevich, Natalia V. Petrova, Daria Onichtchouk

HiChIP to study 3D genome organization in

Xenopus tropicalis embryos / Amina O. Hussein, Ira L. Blitz, Ken W.Y. Cho

CUT&Tag for transcription factor binding profiles during mammalian zygotic genome activation / Wataru Kobayashi, Kikuë Tachibana

Imaging nuclear clusters in live zebrafish embryos / Shivali Dongre, Lennart Hilbert, Nadine L. Vastenbouw

Quantifying transcription factor enrichment at target loci in live embryos / Samantha Fallacaro, Apratim Mukherjee, Ernesto Gamauf, Mustafa Mir

Quantifying nascent transcription in early embryogenesis / Hui Chen

Ribosomal RNA depletion for poly(A)-tail-independent quantification of genome activation / Wesley A. Phelps, Taylor N. Ayers, Miler T. Lee

Monitoring miRNA expression and activity in mammalian oocytes and early embryos / Shubhangini Kataruka, Petr Svoboda

Ribo-ITP : A method for ribosome profiling in single cells and low-input samples / Shilpa Rao, Tori Tonn, Can Cenik

Imaging translation in early embryo development / Pierre Bensidoun, Morgane Verbrugghe, Mounia Lagha

Image segmentation software for nuclear segmentation and cell cycle tracking from 4D embryo light sheet data / Haseeb Khalid Qureshi, Andor Magony, Yavor Hadzhiev, Kacper Wozniak, Attila Sík, Ferenc Müller

Quantitative staging of mid-blastula zebrafish embryos by nuclei counting / William D. Jones, Mary C. Mullins.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed June 4, 2025).

Other Format:

Print version: Zygotic genome activation

ISBN:

9781071645222

1071645226

OCLC:

1522019580

Publisher Number:

CIPO000228349

CIPO000228348

Access Restriction:

Restricted for use by site license