Skin tissue engineering : methods and protocols / edited by Thomas Biedermann and Sophie Böttcher-Haberzeth.

Format:

Book

Contributor:

Biedermann, Thomas, 1979- editor.

Böttcher-Haberzeth, Sophie, 1976- editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 2922.

Methods in molecular biology, 1940-6029 ; 2922

Language:

English

Subjects (All):

Tissue engineering--Laboratory manuals.

Tissue engineering.

Skin--Laboratory manuals.

Skin.

Skin--Cytology.

Genre:

Laboratory manuals

Laboratory manuals.

Physical Description:

1 online resource (xvi, 358 pages) : illustrations (some color).

Edition:

Second edition.

Place of Publication:

New York, NY : Humana Press, [2025]

Summary:

This second volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. Chapters describe the isolation, culture of diverse skin cells, skin models, and managing these cells within co-culture systems. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Skin Tissue Engineering: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.

Contents:

Expansion of human epidermal keratinocytes in co-culture with human feeder cells / Daisuke Suzuki, Filipa Pinto, and Makoto Senoo

Isolation and culture of epidermal melanocytes / Muriel Cario and Alain Taieb

Initiating separate cultures of keratinocytes and melanocytes from a single biopsy / Karel Ferland, Henri De Koninck, Bettina Cattier, Sergio Cortez Ghio, Danielle Larouche, and Lucie Germain

Long-term expansion of mouse primary epidermal keratinocytes using a TGF-ß signaling inhibitor / Filipa Pinto, Daisuke Suzuki, and Makoto Senoo

Isolation and culture of hair follicle dermal sheath mesenchymal stromal cells / Dongrui Ma, Seng-Teik Lee, and Alvin Wen Choong Chua

Isolation and culture of human dermal fibroblasts / Fabiana Ziliani, Katarzyna Michalak-Micka, and Agnes S. Klar

Isolation and culture of human dermal microvascular endothelial cells / Jennifer Bourland, Dominique Mayrand, Nathalie Tremblay, Véronique J. Moulin, Julie Fradette, and François A. Auger

Isolation of stromal vascular fraction by fractionation of adipose tissue / Joeri van Boxtel, Mustafa Uguten, Martin C. Harmsen, Hieronymus P. Stevens, and Joris A. van Dongen

Engineering a pigmented skin equivalent that is responsive to external stimuli / Paola De Los Santos Gomez, Kirsty Goncalves, Victoria Maltman, Lucy Smith, and Stefan Przyborski

Engineering a multilayered skin substitute with keratinocytes, fibroblasts, adipose derived stem cells and adipocytes / Maike Keck, Alfred Gugerell, and Johanna Kober

Isolation and culture of epidermolysis bullosa cells and organotypic skin models / Yinghong He and Cristina Has

Engineering a multilayered skin equivalent : the importance of endogenous extracellular matrix and model flexibility for nuanced and accurate skin studies / Lucy Smith, Victoria Maltman, Paola De Los Santos Gomez, Kirsty Goncalves, and Stefan Przyborski

Bioprinting a tri-layered skin analogue / Hilal Mete Gunaydin, Saskia M. Fogg, Richard J. A. Moakes, and Liam M. Grover

Transdifferentiation of human dermis derived fibroblasts into iNeurons and cultivation in a collagen type 1 hydrogel / Nathalie A. Senn and Thomas Biedermann

Deep proteome profiling of primary skin fibroblasts / Sibilla Sander, Alexandre Leytens, and Jörn Dengjel

Characterization of human skin derived cells by Raman micro-spectroscopy / Luca Pontiggia, Hesham Yosef, Thomas Biedermann, and Ueli Moehrlen

Standard preparation protocol of human skin and skin substitute samples for transmission electron microscopy / Gery Barmettler and Urs Ziegler

Methods for assessing scaffold vascularization with human endothelial cells / Anne M. Kong, Guei-Sheung Liu, Geraldine M. Mitchell, and Shiang Y. Lim

Histological and histochemical methods for the evaluation of tissue engineered skin substitutes / Fernando Campos, Víctor Carriel, Fabiola Bermejo-Casares, Jesús Chato-Astrain, and Miguel Alaminos

Pig model to test tissue engineered skin / Christian Tapking and Ludwik K. Branski

Transplantation of autologous dermo-epidermal skin substitutes in a pig model / Thea Fleischmann, Flora Nicholls, Miriam Lipiski, Margarete Arras, and Nikola Cesarovic

Ex vivo human skin model to study immediate and early local responses to external stimuli / Ines Foessl, Thomas Birngruber, Petra Kotzbeck, and Elisabeth Hofmann

A method to evaluate skin and skin substitute mechanical properties using a suction device / Vivienne Woodtli, David Sachs, Alberto Stracuzzi, Serjosha Robmann, Thomas Biedermann, Sophie Böttcher-Haberzeth, and Bettina Thumm

GMP compliant production of therapeutic components of autologous adipose tissue / Mustafa Uguten, Joeri van Boxtel, Hieronymus P. Stevens, Martin C. Harmsen, and Joris A. van Dongen

Quality controls of autologous skin products according to European GMP guidelines / Rocio García de la Cruz Valencia, Gloria Carmona Sánchez, Céline Auxenfans, Jean-François Brunet, Ana Fernández González, Vivienne Woodtli, Alexandra Karström, and Marina Trouillas

Standardized outcome measures for the clinical application of tissue engineered products / Anna S. van den Bosch, Melinda Farkas, Frederique M. Kemme, Matthea M. Stoop, Paul P. M. van Zuijlen, Annebeth Meij-de Vries, Esther Middelkoop, and Anouk Pijpe.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed April 15, 2025).

Other Format:

Print version: Skin tissue engineering.

ISBN:

9781071645109

1071645102

OCLC:

1514956098

Publisher Number:

CIPO000228343

Access Restriction:

Restricted for use by site license