Advanced cell and molecular techniques protocols for in vitro and in vivo studies Karuppusamy Arunachalam, Puthanpura Sasidharan Sreeja

Format:

Book

Author/Creator:

Arunachalam, Karuppusamy, author.

Sreeja, Puthanpura Sasidharan, author.

Series:

Springer protocols (Series) 1949-2456

Springer Protocols Handbooks 1949-2456

Language:

English

Subjects (All):

Cytology--Technique.

Cytology.

Cytology--Research.

Molecular biology--Technique.

Physical Description:

1 online resource

Place of Publication:

Springer US 2025

New York, NY Humana Press 2025

Summary:

This detailed volume explores laboratory protocols and methodologies for studying cell cultures, genetic analysis, and various biochemical assays. The chapters within this book cover a wide range of techniques, from basic cell culture methods to more specialized assays used in the study of genotoxicity, apoptosis, and gene expression, not only relevant to academic researchers but also to professionals in the pharmaceutical, biotechnological, and clinical fields. Written for the Springer Protocols Handbooks series, each protocol has been carefully compiled to provide step-by-step instructions, ensuring clarity and reproducibility. Authoritative and practical, Advanced Cell and Molecular Techniques: Protocols for In Vitro and In Vivo Studies serves as both a hands-on guide and a source of inspiration for future research as the fields of molecular biology and toxicology continue to evolve

Contents:

Cell Culture Techniques

Solutions Preparation

Standard Operating Procedure (SOP) for Maintaining Sterility in the Laminar Flow Hood During Cell Culture Procedures

Protocol for Maintenance, Isolation, and Primary Culture of Ehrlich Tumor Cells

Stem Cell Cultivation and Maintenance

Protocol for Isolation of Stem Cells from Rabbit Adipose Tissue

Protocol for Isolation of Lung Fibroblasts from Mouse Tissue

Protocol for Isolation of Mice Fat Stem Cells

Protocol for Isolation of Mouse Adipose-Derived Stem Cells (ADSCs)

Protocol for Isolation of Bone Marrow Stem Cells

Extraction of Mesenchymal Stem Cells (MSCs) from Human Liposuction Fat

Human Bone Marrow Extraction Protocol

Mesenchymal Stem Cells (MSCs) Differentiation Protocols

cDNA Synthesis and Quantitative PCR (qPCR) for Gene Expression Analysis

Preparation and Optimization of Reagents for Genetic Analysis and Infectious Disease Diagnosis

DNA and RNA Extraction from Plasma Using Magnetized Silica

RNA Extraction Using Trizol (6-Well Plate Method)

Agarose Gel Electrophoresis for RNA Integrity Assessment

DNA Purification Using the Wizard Plus SV Minipreps DNA Purification System (Promega)

Detection of Reactive Oxygen Species (ROS) in Embryos

Determination of Protein Concentration: Sedmak and Grossberg Method

Cell Sorting of Fluorescent Zebrafish Cells

Cell Disaggregation for Flow Cytometry Analysis of Fluorescent Zebrafish Cells

RNA Extraction from Zebrafish Embryos for RT-qPCR

Extraction of Genomic DNA

RNA Extraction from HEK293

Agarose Gel Preparation for RNA and DNA Electrophoresis

Zebrafish Genotyping by Fin Clipping

In Situ Hybridization of Zebrafish Embryos

Concepts and Practices in Flow Cytometry

In Situ Apoptosis Assay

Flow Cytometry Protocols for Assessing Membrane Integrity, Cell Cycle, and Apoptosis in Treated Cells

Leukocyte Reading Protocol on the Flow Cytometer

Flow Cytometry Protocol for Mouse Sperm Cells (BD Accuri C6+)

Extraction of Yolk Proteins from 24 HPF Zebrafish Embryos

PFA Fixation for Zebrafish Embryos

Thioflavin T (ThT) Fluorescence Measurement

Alcian Blue Staining Protocol

Zebrafish Embryo Staining with Nicotinamide Adenine (NA)

Western Blot on Zebrafish Embryos

In Vitro Micronucleus Assay in Binucleated CHO-K1 Cells

In Vitro Assessment of Genotoxicity Using the Comet Assay in CHO-K1 Cells

Treatment and Cell Collection for Chromosomal Aberration Test

Electrophoretic Mobility Shift Assay (EMSA): Non-Radioactive, Band Shift, Gel Shift, or Gel Retardation

MTT Assay Protocol

Spectropolarimeter (Jasco-1500) Operation Protocol

Immunoprecipitation Protocol (Tuning)

qPCR EvaGreen Hot FIREPol Mix

Synthesis of RNA-Digoxigenin Probe

Thermal Difference Spectra (TDS)

Generation of Competent Bacteria

Retro Transcription via STEM LOOP

In Vitro Transcription with the mMessage mMachine SP6 Transcription Kit

Transfection in HEK293 Cells

HeLa Transfections with Lipofectamine 3000

Chemical Competent Cell Transformation

Notes:

Includes author index

Online resource; title from PDF title page (SpringerLink, viewed May 1, 2025)

Other Format:

Print version :

ISBN:

9781071645185

1071645188

OCLC:

1517964387

Publisher Number:

CIPO000228347

Access Restriction:

Restricted for use by site license