Zymography : biological and clinical applications. Volume 2 / edited by Raouf A. Khalil.

Format:

Book

Contributor:

Khalil, Raouf A., editor.

Series:

Methods in molecular biology (Clifton, N.J.)

Methods in molecular biology, 1940-6029

Language:

English

Subjects (All):

Enzymes--Analysis--Laboratory manuals.

Enzymes.

Enzyme activation--Laboratory manuals.

Enzyme activation.

Enzymes--Analysis.

Genre:

Laboratory manuals

Physical Description:

1 online resource (xvi, 280 pages) : illustrations (some color).

Place of Publication:

New York, NY : Humana Press, 2025.

Summary:

"Volume 2 describes how to determine the activity of different isozymes, allozymes, and families of proteinases to advance the fields of enzymology and molecular evolution, and provides useful biomarkers for various biological processes, pathological conditions, and clinical disorders. The chapters in Volume 2 are organized in three parts. Part I introduces in situ zymography and localization of bright green-fluorescent gelatinase activity in tissue sections, in situ zymography in formalin-fixed paraffin-embedded and mineralized tissues, and in vivo zymography as an essential activity assay for studying the activity of matrix metalloproteinases (MMPs) in a cell-specific manner in the brain. Part II focuses on biological applications of zymography such as fundamentals of zymography and its applications to the study of biological samples, gelatin zymography to quantify MMP-2 and MMP-9 in complex biological specimens, and detection of proteolytic enzymes in polyacrylamide gels supplemented with diverse biological substrates. Part III focuses on potential clinical applications of zymography, with chapters describing assessment of MMP-2 and MMP-9 hydrolytic activity in preclinical and clinical tissue samples, the use of zymography to assess circulating MMP-2 and MMP-9 in plasma and serum and in pathological conditions, and the use of zymography for the detection of bacterial proteases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls"-- Provided by publisher.

Contents:

In situ zymography and localization of bright green fluorescent gelatinase activity in tissue sections / Michele M. Castro, Raouf A. Khalil

In situ zymography in formalin-fixed paraffin-embedded and mineralized tissues / Juliana De Lima Gonçalves, Nilza Letícia Magalhães, Alexandra Mussolino De Queiroz, Francisco Wanderley Garcia Paula-Silva

In vivo zymography : An essential activity assay for studying the activity of matrix metalloproteinases in specific brain cells / Ritchy Hodebourg

Fundamentals of zymography : Applications to the study of biological samples / Jorge Recio-Aldavero, Laura Muñoz-Moreno, I. Dolores Román, Ana M. Bajo

Gelatin zymography to quantify levels of MMP-2 and MMP-9 in complex biological samples / John Aaron Howell, Eduardo Candelario-Jalil

Detection of proteolytic enzymes in polyacrylamide gels supplemented with diverse biological substrates / Martin E. Argañaraz, Mariela Roldán-Olarte

Evaluating matrix metalloproteinase expression in primary mouse glial cultures using gel zymography / Arjun Sapkota, Sebok K. Halder, Richard Milner

Zymography as a tool for exploring protease activity in dental research / Polliana Mendes Candia Scaffa, Marília Afonso Rabelo Buzalaf

Zymography techniques for the profiling of digestive protease in a freshwater invertebrate model / Federico A. Dellagnola, Martín S. Godoy, Israel A. Vega

Casein zymography : A rapid and robust methodology for detecting calpain protease activity in meat product / María Sumampa Coria, Gabriela Maria Grigioni

Cost-effective method for the detection of lipases and proteases in crude extracts of microbial proteins using gel zymography / Fabiola Altimira, Matías Arias-Aravena, Eduardo Tapia

Applying zymography methods for the detection of enzymes from fungal endophytes / Trung Q. Do, Enzo A. Palombo, Bita Zaferanloo

Assessing MMP-2/9 proteolytic activity and activation status by zymography in preclinical and clinical tissue samples / Carolane Veilleux, Marie-Eve Roy, Borhane Annabi

Using zymography to assess circulating matrix metalloproteinase (MMP)-2 and MMP-9 in clinical samples / Sandra O. Conde-Tella, Raquel F. Gerlach, Jose E. Tanus-Santos

Zymography : A simple and powerful tool for the assessment of MMP-2 and MMP-9 in pathological conditions / Joseph George

Zymography for the detection of bacterial proteases / Mary E. Marquart

Gelatin zymography of proteases in chronic wound samples / Vojtěch Pavlík, Aneta Horčičková

In-gel zymography of amyotrophic lateral sclerosis-associated variants of superoxide dismutase-1 / Jeremy S. Lum, Mikayla L. Brown, Siobhan C. Suters, Justin J. Yerbury, Luke McAlary

Assessing gelatinase activity in normal and disease uterine tissue and cells via gelatin zymography / Riley Peterson, Warren B. Nothnick

Gelatin zymography of conditioned media from tumor cell lines / Adriane S. De Siqueira, Maria S. Da S. Kataoka, Sérgio M. Alves Junior, Vanessa M. Freitas, Ruy G. Jaeger, João J.V. Pinheiro

Analysis of metalloproteinase secretion in cancer cells by zymography / Javier De Jesus Vasconcelos-Ulloa, Jesus Omar Medina-Cruz, Octavio Galindo-Hernandez

Evaluating matrix metalloproteinase activity in human hepatocellular carcinoma tissues and cells with gelatin zymography / Diwakar Suresh, Amith Bharadwaj, Prasanna K. Santhekadur, Divya P. Kumar

Gelatin zymography : Detecting gelatinase activity as a partner for invadopodia-mediated invasion by tumor cells / Marija Dinevska, Samuel Widodo, Chantel McEntee, Theo Mantamadiotis, Stanley S. Stylli.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed April 30, 2025).

Other Format:

Print version: Zymography

ISBN:

9781071644829

1071644823

OCLC:

1517870506

Publisher Number:

CIPO000208852

CIPO000208853

Access Restriction:

Restricted for use by site license