T cell activation : methods and protocols / edited by Björn-Philipp Diercks.

Format:

Book

Contributor:

Diercks, Björn-Philipp, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 2904.

Springer protocols (Series)

Methods in molecular biology, 1940-6029 ; 2904

Springer protocols

Language:

English

Subjects (All):

T cells--Laboratory manuals.

T cells.

T cells--Research.

Genre:

Laboratory manuals

Physical Description:

1 online resource (xii, 274 pages) : illustrations (some color).

Place of Publication:

New York, NY : Humana Press, [2025]

Summary:

"This volume provides detailed, up-to-date methods used in basic and applied research on T-cell activation. Chapters explore the fundamentals in T-cell biology a board range cutting-edge techniques from initial Ca2+ live-cell imaging to downstream effector functions in autoimmune disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, T- cell activation: Methods and Protocols will be an indispensable protocol collection for researchers studying T-cell activation in inflammation and immunity"-- Provided by publisher.

Contents:

Imaging protocol for super-resolution microscopy in fixed t cells / Miriam Kopdag, Lola C. Hernandez, Anette Rosche, and Björn-Philipp Diercks

Visualization and quantification of NFAT1 translocation in t cells / Franziska Möckl, Dejan Kovacevic, René Werner, Björn-Philip Diercks, and Mariella Weiß

Deep learning-based image restoration and super-resolution for fluorescence microscopy : Overview and resources / David Lohr, Lina Meyer, Lena-Marie Woelk, Dejan Kovacevic, Björn-Philipp Diercks, and René Werner

Analyzing point accumulation in nanoscale topography (PAINT) data to decipher t cell receptor cluster signalling / Shirin Ansari, James C. Walsh, and Jesse Goyette

Measuring NAADP-evoked Ca2+ release in permeabilized t cells / Kai J. Winterberg, Andreas Bauche, Ralf Fliegert, and Andreas H. Guse

An improved two-dimensional HPLC method for endogenous 2'-deoxy-ADPR and 2'-deoxy-NAD / Feng Gu, Ralf Fliegert, Andreas Bauche, and Andreas H. Guse

Analysis of store-operated Ca2+ entry in primary t cells / Sara T. Granados, Sergei Yanushkevich, Jessica Lok, and Axel R. Concepcion

Monitoring dynamic of CAMP signaling in primary murine CD4+ t cells via live-cell imaging / Roberta Kurelić, Marie Bielenberg, and Viacheslav O. Nikolaev

Using specific reporter mice to assess CD4+ t cell transdifferentiation / Mikolaj Nawrocki and Samuel Huber

Generation of antigen-specific CD4+ primary rat t cell lines / Lukas C. Müller-Kirschbaum, Dmitri Lodygin, Francesca Odoardi, and Arianna Merlini

Induction and assessment of activation in rat effector CD4+ memory th cells / Lukas C. Müller-Kirschbaum, Dmitri Lodygin, Francesca Odoardi, and Arianna Merlini

Imaging BiTE-mediated activation of primary human CD8+ t cells / Kai J. Winterberg, Constantin Möller, Lea I. Schwarze, Sophia Cichutek, Julia Hambach, Andreas H. Guse, Boris Fehse, Björn-Philipp Diercks, and Kristoffer Riecken

Flow cytometric analysis of mouse brain CD8+ t cell activation / Du Huy Nguyen, Manja Wibke Thiem, Alexander Winston Veltkamp, Ines Sophie Schädlich, Jiani Jiang, Mamta Panda, Enrico Maria Costanzo, Tim Magnus, and Björn Rissiek

Utilizing pMHC multimers to study antigen-specific t cells and t cell exhaustion in chronic viral infections / K. Keetz, M. Knapp, and J. Schulze zur Wiesch

Analysis of TF/DNA binding in t cells applying alpha technology / Nikolaus Thuille and Gottfried Baier

Using seahorse technology as an efficient way of verifying t cell stimulation / Jonas Aakre Wik, Kristine Stromsnes, and Bjørn Steen Skålhegg

Metabolic screening of t lymphocytes during activation via SEAHORSE extracellular flux (XF) analysis / Gabriela F. Gubert, Sophia M. Hochrein, Katrin Sinning, and Martin Vaeth

Metabolic profiling of activated t lymphocytes using single-cell energetic metabolism by profiling translation inhibition (SCENITH) / Katrin Sinning, Sophia M. Hochrein, Gabriela F. Gubert, and Martin Vaeth.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed April 15, 2025).

Other Format:

Print version: T cell activation

ISBN:

9781071644140

1071644149

OCLC:

1514955704

Publisher Number:

CIPO000228338

Access Restriction:

Restricted for use by site license