Synapse development : methods and protocols / edited by Tolga Soykan, Alexandros Poulopoulos.

Format:

Book

Contributor:

Soykan, Tolga, editor.

Poulopoulos, Alexandros, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 2010.

Springer protocols (Series)

Methods in Molecular Biology, 1940-6029 ; 2010

Springer protocols

Language:

English

Subjects (All):

Synapses--Laboratory manuals.

Synapses.

Genre:

Laboratory manuals

Physical Description:

1 online resource (xii, 278 pages) : illustrations (some color).

Edition:

Second edition.

Place of Publication:

New York, NY : Humana Press, [2025]

Summary:

"This fully updated volume brings together synapse development methods that span from the macroscopic to the nanoscale in order to foster novel perspectives and encourage interdisciplinary integration. Beginning with a section on in vitro formation and manipulation of synapses, including cutting-edge approaches for neuron differentiation and genomic modification, the book continues by covering methods for dissecting synaptic components at high resolution, utilizing advanced imaging and purification techniques, as well as approaches for studying synaptic networks and activity patterns in their native contexts, featuring methods for labeling, tracing, and quantifying components of developing neural circuits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Synapse Development: Methods and Protocols, Second Edition serves as valuable resource for researchers seeking to advancing our understanding of how synapses form, transform, and transmit"-- Provided by publisher.

Contents:

Synaptogenic assays using primary neurons cultured on micropatterned substrates / Katalin Czöndör, Nathalie Piette, Béatrice Tessier, Vincent Studer, and Olivier Thoumine

Generation of glutamatergic human neurons from induced pluripotent stem cells / Filiz Sila Rizalar and Volker Haucke

Generation of prefrontal cortex neurons from human pluripotent stem cells under chemically defined conditions / Gustav Y. Cederquist, Polina Oberst, Xuyao Chang, Jason Tchieu, and Lorenz Studer

Differentiation of mature dopaminergic neurons from human induced pluripotent stem cells / Pretty Garg, Mathias Bähr, and Sebastian Kügler

Prime editing of mouse primary neurons / Colin D. Robertson, Ryan R. Richardson, Marilyn Steyert, Corinne A. Martin, Corey Flynn, and Alexandros Poulopoulos

Purification of afference-specific synaptosome populations using fluorescence-activated synaptosome sorting / Vincent Paget-Blanc, Marie Pronot, Marlene E. Pfeffer, Maria Florencia Angelo, and Etienne Herzog

Investigating the molecular composition of neuronal subcompartments using proximity labeling / Mareike Lohse, Siqi Sun, Maksims Fiosins, Stefan Bonn, Pedro Zamorano, Olaf Jahn, and Noa Lipstein

Monitoring synapses via trans-synaptic GFP complementation / Theodoros Tsetsenis

Imaging synapse ultrastructure and organization with STED microscopy / Marta Maglione and Stephan J. Sigrist

Photomarking relocalization technique for correlated two-photon and electron microscopy imaging of single stimulated synapses / Miquel Bosch, Jorge Castro, Mriganka Sur, and Yasunori Hayashi

Image analysis routines for quantification of synaptic density and connectivity / Luca Della Santina and Mrinalini Hoon

Monosynaptic tracing in developing circuits using modified rabies virus / Laura Cocas and Gloria Fernandez

Gene knockout in the developing brain of wild-type rodents by CRISPR in utero electroporation / Andrea J. Romanowski, Ryan R. Richardson, Celine Plachez, Reha S. Erzurumlu, and Alexandros Poulopoulos

Mapping synaptic activity at the population and cellular levels with genetically encoded voltage indicators (GEVIs) / Younginha Jung, Ryuichi Nakajima, Sung Min Ahn, Nazarii Frankiv, Haeun Lee, Maesoon Im, Yoon-Kyu Song, and Bradley J. Baker

Visualization of synapses in larval stages of Drosophila melanogaster using the GRASP technique / Claudia Gualtieri and Fernando J. Vonhoff

Sparse labeling, rapid clearing, and native fluorescence light sheet imaging in the developing rodent cerebellum / Cheryl Brandenburg and Alexandros Poulopoulos.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed April 15, 2025).

Other Format:

Print version: Synapse development.

ISBN:

9781071644461

1071644467

OCLC:

1514955480

Publisher Number:

CIPO000224112

Access Restriction:

Restricted for use by site license