Spermatology : methods and protocols / edited by Manuel Álvarez-Rodríguez.

Format:

Book

Contributor:

Álvarez Rodríguez, Manuel, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 2897.

Methods in molecular biology, 1940-6029 ; 2897

Language:

English

Subjects (All):

Spermatozoa--Laboratory manuals.

Spermatozoa.

Semen--Laboratory manuals.

Semen.

Physical Description:

1 online resource (xxii, 713 pages) : illustrations (some color).

Place of Publication:

New York, NY : Humana Press, [2025]

Summary:

This volume covers a broad range of procedures and protocols that discuss the steps for designing experimental setups for studying sperm, including analytical tools for diverse species. The chapters in this book are organized into 5 parts: Part One looks at the latest classical methods of analysis of sperm quality parameters; Part Two discusses the process of sperm collection in wild and domestic species; Part Three covers spermatozoa, including cryopreservation and analysis of a variety of sperm characteristics; Part Four focuses on molecular determinants in seminal plasma; and Part Five talks about sperm interactions, describing the fine cross-talk of the male gametes with the female genital tract. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Spermatology: Methods and Protocols is a valuable resource for both novel and expert researchers who want to learn more about sperm-related methodologies.

Contents:

Spermatology : current methods to study sperm classical variables / Alejandro Vicente-Carrillo, Eduardo de Mercado de la Peña, Adrián Martín San Juan, Helena Nieto-Cristóbal, Heriberto Rodríguez-Martínez, and Manuel Álvarez-Rodríguez

Evolutionary aspects of sperm physiology and its assessment / Eduardo R. S. Roldan

Assessment of boar semen morphology and morphometry / Joaquín Gadea

Semen extraction and analysis in domestic animals / Ana Josefa Soler, Olga García-Álvarez, Juan Angel Laborda-Gomariz, Pilar Jiménez-Rabadán, María Iniesta-Cuerda, Alejandro Maroto-Morales, Manuel Ramón, Vidal Montoro, María del Rocío Fernández-Santos, and J. Julián Garde

Semen extraction and management in wild animals : TUMASG technique / Julián Santiago-Moreno, Adolfo Toledano-Díaz, Cristina Castaño, and Rodolfo Ungerfeld

Semen extraction and analysis in avian species / Bushra A. Rakha, Sumiyyah Zuha, and Shamim Akhter

Boar semen assessment for the production of artificial insemination doses / Dagmar Waberski and Martin Schulze

Semen evaluation in the production of bovine artificial insemination doses / Leonardo F. C. Brito

Sperm vitrification in horse and donkey / Manuel Hidalgo and Isabel Ortiz

Cryopreservation of dog semen / Jesús Dorado and Isabel Ortiz

Rabbit sperm cryopreservation / Maria Pilar Viudes de Castro, Francisco Marco-Jimenez, and Jose Salvador Vicente

Cryopreservation of small ruminant sperm and detection of sperm cryocapacitation / Patricia Peris-Frau and Silvia Gimeno-Martos

Sperm cryopreservation in boars / Inmaculada Parrilla, María A. Gil, Cristina Cuello, and Emilio A. Martinez

Cryopreservation of horse sperm / Jordi Miró, Iris Martínez-Rodero, Marc Yeste, and Jaime Catalán

New delivery systems on sperm quality improvement / Alejandro Jurado-Campos, Pedro Javier Soria-Meneses, María Arenas-Moreira, Carlos Alonso-Moreno, Ivan Bravo-Pérez, Ana Josefa Soler, José Julián Garde, and María del Rocío Fernández-Santos

Assessment of sperm motility with the use of computer-aided sperm analysis (CASA) / Gerhard van der Horst and Liana Maree

Bioinformatics on sperm subpopulations using computer-assisted sperm analysis (CASA) / Felipe Martínez-Pastor and Manuel Ramón

Sperm selection by colloid centrifugation / Jane M. Morrell

Bioenergetics of rodent spermatozoa / Eduardo R. S. Roldan, Maximiliano Tourmente, Ana Sanchez-Rodriguez, and Eduardo Rial

Flow cytometry in the assessment of sperm function parameters : viability, acrosomal integrity, membrane stability, and mitochondrial status / Nana Satake and Gry Boe-Hansen

Dextran/swim-up procedure for ram sperm selection / Rosaura Pérez-Pé, Teresa Muiño-Blanco, José Álvaro Cebrián-Pérez, and Adriana Casao

Chromatin structure assessment using flow cytometry after DNA denaturation and acridine orange staining / Felipe Martinez-Pastor

Assessment of the sperm DNA oxidative damage by immunofluorescence and flow cytometry / Estíbaliz Lacalle, Estela Fernández-Alegre, Belén Gómez-Giménez, Felipe Martínez-Pastor, and Cristina Soriano-Úbeda

Sperm intracellular calcium evaluation / Adriana Casao, Sara Miguel-Jimenez, and Rosaura Pérez-Pé

Oxidative status measurement in domestic and wild small ruminants’ species / Olga García-Álvarez, Ana Josefa Soler, María Iniesta-Cuerda, María del Rocío Fernández-Santos, Felipe Martínez-Pastor, Enrique del Olmo, Pedro Javier Soria-Meneses, Alejandro Jurado-Campos, Alejandro Maroto-Morales, Patricia Peris-Frau, Vidal Montoro, and Jose Julián Garde

Evaluation of oxidative stress in mammalian spermatozoa / Zamira Gibb, Róisin A. Griffin, Elizabeth G. Bromfield, Sarah R. Lambourne, Alexandra Wilkins, Ceilidh Jenkins, Aleona Swegen, R. John Aitken, and Geoffry N. De Iuliis

Real-time acrosome reaction and intracellular calcium recordings in mice sperm / Claudia Sánchez Cárdenas, Arturo Hernández-Cruz, Pablo E. Visconti, and Alberto Darszon

Field emission scanning electron microscopy (FE-SEM) as an approach for membrane surface mapping in cell biology / Laura Robles-Gómez, Paula Sáez-Espinosa, and María José Gómez-Torres

Detection of sperm membrane proteins by immunocytochemistry, scanning and transmission electron microscopy, and Western blotting / Elisabeth Pinart

Detection and quantification of mRNA in mammalian spermatozoa / Won-Ki Pang and Myung-Geol Pang

Techniques to determine mammalian sperm capacitation / Joan E. Rodríguez Gil and Olga Blanco-Prieto

Disulfide bridges assessment in the sperm chromatin by flow cytometry / Felipe Martínez-Pastor, Belén Gómez-Giménez, and Cristina Soriano-Úbeda

Analysis of the chromatin structure by chromomycin A3 (CMA3) and flow cytometry / Belén Gómez-Giménez, Estíbaliz Lacalle, Felipe Martínez-Pastor, and Cristina Soriano-Úbeda

Isolation of high-quality RNA from mammalian spermatozoa for transcriptome studies / Bijayalaxmi Sahoo and Mukesh Kumar Gupta

Prediction of circRNAs in mammalian spermatozoa from RNA sequencing data / Bijayalaxmi Sahoo and Mukesh Kumar Gupta

Reduced representation bisulfite sequencing library preparation for sperm DNA methylation analyses : manual and automated methods / Jean-Philippe Perrier, Aurélie Chaulot-Talmon, Francesca Ali, Laurent Schibler, Hélène Jammes, and Hélène Kiefer

Production of healthy mice from freeze-dried spermatozoa preserved at room temperature / Sayaka Wakayama, Daiyu Ito, and Teruhiko Wakayama

High-throughput analysis of postmeiotic male germ cells using image flow cytometry / Maria Eugenia Teves, Eduardo R. S. Roldan, Clara Agudo-Rios, Julie Farnsworth, and Larry Boyle

Assessment of bacterial contamination in semen / Ferran Garriga, Sergi Bonet, and Marc Yeste

A size-exclusion chromatography-based procedure for isolating extracellular vesicle subsets from porcine seminal plasma / Pablo Martínez-Díaz, Ana Parra, Christian M. Sanchez-López, Antonio Marcilla, Diego Bucci, Jordi Roca, and Isabel Barranco

Molecular determinants in pig semen : verified protocols for cytokine detection and quantification / Isabel Barranco and Cristina A. Martinez

Molecular determinants in seminal plasma and spermatozoa : nontargeted metabolomics / André Furugen Cesar de Andrade, Mariana Andrade Torres, Heidge Fukumasu, Arina Lázaro Rochetti, Simone Maria Massami Kitamura Martins, Francisco José de Novais, Christina Ramirez, and Bruce Cooper

Seminal plasma proteomics using filter-aided sample preparation / Taylor Pini and Simon P. de Graaf

Assessing seminal fluid interaction with female reproductive tract tissues : in vivo and in vitro models for mouse / Hon Yeung Chan, John E. Schjenken, David J. Sharkey, and Sarah A. Robertson

Seminal fluid interaction with female reproductive tract epithelial cells : in vitro models for human / David J. Sharkey, Hannah E. Lyons, Hon Yeung Chan, and Sarah A. Robertson

Impact of seminal plasma on the embryonic development and uterine environment in pigs / Emilio A. Martinez, Cristina Cuello, Maria A. Gil, and Inmaculada Parrilla

Assessment of sperm binding capacity in the reservoir using an in vitro model of oviduct epithelial spheroids in cattle / Coline Mahé, Ludivine Laffont, Thanya Pranomphon, Lorraine Schmaltz, Karine Reynaud, Pascal Mermillod, and Marie Saint-Dizier

Characterization of ovine cervical mucus and implementation of a sperm–mucus penetration test using fluorescently labeled sperm / Laura Abril-Parreño, Kaitlyn Weldon, and Sean Fair.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (Springer Nature Link, viewed March 4, 2026).

Other Format:

Print version: Spermatology

ISBN:

9781071644065

1071644068

OCLC:

1514956028

Publisher Number:

CIPO000224110

Access Restriction:

Restricted for use by site license