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The UFAW handbook on the care and management of laboratory and other research animals / edited by Huw Golledge, Claire Richardson.
- Format:
- Book
- Series:
- UFAW animal welfare series
- UFAW Animal Welfare Series
- Language:
- English
- Subjects (All):
- Laboratory animals--Handbooks, manuals, etc.
- Laboratory animals.
- Physical Description:
- 1 online resource
- Edition:
- Ninth edition.
- Other Title:
- Universities Federation for Animal Welfare handbook on the care and management of laboratory and other research animals
- Place of Publication:
- Hoboken, NJ : Wiley-Blackwell, 2024.
- Contents:
- Cover
- Title Page
- Copyright Page
- Contents
- List of contributors
- Foreword
- Chapter 1 Introduction
- PART 1 IMPLEMENTING THE THREE RS IN RESEARCH USING ANIMALS
- Chapter 2 The Three Rs
- Opening remarks
- Introduction
- Simple words, complex meanings
- The origin and evolution of the Three Rs
- A holistic approach to the Three Rs
- Replacement
- Replacement strategies
- Replacement methods
- The high-fidelity fallacy
- Reduction
- Experimental design
- Re-use of animals
- Optimising animal production
- Refinement
- Assessing animal well-being
- Severity scoring systems
- Observation schedules
- Contingent and direct harms
- Humane endpoints
- Responsibility for the Three Rs
- The individual
- The institution
- Funding bodies
- Scientific journals
- International and national efforts
- Concluding remarks
- References
- Chapter 3 The design of animal experiments
- The research strategy
- Choosing the animal and procedure
- Defining the purpose of the experiment
- The decision-making process
- Variation among the animals
- Steps in the design of an experiment
- Defining which dependent variables to measure
- Defining which effects to investigate
- Identifying the experimental unit
- Determining the sample size (replication of the experimental units)
- Assessing the measurement and procedural variability
- Choosing an experimental design
- Randomising the experiment
- Blinding the experiment
- Performing the statistical analysis
- Reporting the results of the experiment
- Types of experimental designs
- The completely randomised design
- The randomised block design
- The Latin square design
- The cross-over design
- The factorial design
- The repeated measures design
- Types of statistical analysis
- ANOVA and the t-test
- Analysis of covariance.
- The chi-squared and Fisher's exact tests for discrete data (counts)
- The non-parametric tests
- Summary
- Acknowledgements
- Chapter 4 An introduction to laboratory animal genetics
- Molecular genetics
- DNA - the molecule of inheritance
- Protein-coding DNA
- Gene structure
- Chromosomes
- Genes and alleles
- Protein synthesis
- Regulatory DNA and cell-specificity
- Other non-coding DNA
- Mutations and variation
- Genetics and the laboratory animal
- Gene nomenclature
- Inbred strains
- Genetic drift
- Substrains
- Maintaining inbred strains
- Characteristics of an inbred line
- Selecting an appropriate inbred strain
- Inbred strains of other species
- Outbred strains
- GA laboratory animals
- Terminology
- Spontaneous mutations
- Inducing mutations in laboratory animals
- Gene editing - a revolution for the generation of GA animal models
- Chapter 5 Phenotyping of genetically modified mice
- The welfare of GM animals
- Welfare assessment
- Phenotypic characterisation
- Systematic, broad-based phenotyping
- Early phenotyping protocols
- The Mouse Phenome Project
- Eumorphia
- From EUMODIC to IMPC
- Hypothesis-driven phenotyping
- Before phenotyping - search for information
- Testing for new traits - the hierarchical approach
- Phenotyping - how far do you take it?
- Issues with conventional phenotyping in mice
- Automated in-cage behavioural phenotyping
- Phenotyping challenges - the environment
- Phenotyping challenges - other issues
- Age and sex
- Genetics
- Controls
- Number of animals
- Scientific reporting
- Acknowledgement
- Chapter 6 Brief introduction to welfare assessment: a 'toolbox' of techniques
- Introduction.
- Basic principles of welfare assessment
- Considerations when assessing welfare
- The 'welfare assessment toolbox'
- Routine welfare monitoring
- Assessing welfare and changes in welfare: physiological measures
- Assessing welfare and changes in welfare: behavioural measures
- Assessing welfare and changes in welfare: brain measures
- Assessing animals' preferences and motivation for resources
- Consumer demand
- Assessing pain
- Chapter 7 Welfare and 'best practice' in field studies of wildlife
- Reasons for wildlife research
- Welfare impacts of wildlife studies
- Effects of stress
- Injuries
- Other welfare effects
- The three Rs and welfare
- Capture, handling, release
- General welfare issues
- Capture
- Handling
- Anaesthesia
- Sampling
- Marking
- Release
- To treat or not to treat?
- Legislation appropriate to wildlife studies
- Legislation relating to the use of animals in research
- Wildlife and Countryside Acts
- Other wildlife legislation
- Licences
- International legislation
- Best practice' guidelines
- UK
- Worldwide
- Chapter 8 Legislation and oversight of the conduct of research using animals: a global overview
- History
- Principles
- The Three Rs
- International Guiding Principles for Biomedical Research Involving Animals
- The World Organisation for Animal Health (OIE)
- Engineering versus performance standards
- Adequate veterinary care
- Training and competence
- Institutional and governmental review, oversight and authorisation of animal activities
- Inspection and compliance
- International accreditation of animal care and use programmes
- Regional and international harmonisation of guidelines
- Europe.
- Harmonisation in the region
- From ETS 123 to Directive 2010/63/EU
- Levels of authorisation to work with animals
- Other important aspects of the European framework
- North America
- Canada
- The United States
- Asia
- Japan
- Republic of Korea
- People's Republic of China
- Taiwan, ROC
- India
- Singapore
- Australia and New Zealand
- Australia
- New Zealand
- Examples of oversight elsewhere in the world
- Latin America
- Russian Federation
- Israel
- South Africa
- Chapter 9 Planning, design and construction of the modern animal facility
- Overriding requirements for all research animal facilities
- Project brief
- Types of animal research facility
- Facility accommodation factors
- Legislation
- Procurement models
- Project procurement strategy
- Contractor procurement models
- The client team
- The role of user advisors
- Documentation of the User Requirement Specification (URS)
- Cost consultants
- Budget
- Value engineering (VE)
- The design process
- Project stages as defined by RIBA
- The project team
- Project manager (PM)
- Design team
- Architect
- Services engineer (mechanical, electrical and plumbing services - MEP)
- Fire protection engineer (FP)
- Structural engineer (SE)
- Construction team
- Main contractor
- The sub-contractors
- The suppliers
- Heating, ventilation and air conditioning (HVAC)
- Building management system (BMS)
- Specifications
- Prescriptive specifications
- Performance specifications
- Commissioning
- Pre-handover demonstration of facility reliability
- Verification, validation, stability, reliability
- FRT process
- System/Equipment failure
- Special requirements for research animal facilities
- Environment
- Barrier integrity
- Acceptance of FRT data
- Soft Landings
- Conclusions
- References.
- Chapter 10 Environmental enrichment: animal welfare and scientific validity
- Approaches to enrichment
- Why is enrichment needed?
- Animal behaviour and enrichment in captive environments
- Behaviour in captivity associated with poor welfare
- Behaviour in captivity associated with good welfare
- Effect of environmental enrichment on behaviour and welfare
- Effects of enrichment on experimental outcomes
- Bias and internal validity
- Precision and test sensitivity
- External validity and replicability
- Factors to consider when choosing enrichment
- What are the benefits of the proposed enrichment?
- Enrichment and animal safety
- Enrichment and human safety
- Enrichment and the experiment
- Validating new enrichment
- Managing an enrichment programme
- Chapter 11 Special housing arrangements
- Types of containment
- Protective clothing
- The animal room
- Flexible film isolators
- Rigid isolators
- Individually ventilated cages
- Filter-top cages
- Ventilated cabinets
- Laminar flow booths or cubicles
- Bespoke systems
- Species
- Mice
- Rats
- Hamsters
- Guinea pigs
- Rabbits
- Ferrets
- Nonhuman primates
- Welfare considerations with the use of containment systems for nonhuman primates
- Pigs
- Ruminants
- Birds
- Checklist for containment
- Legislative requirements
- The future
- Chapter 12 Transportation of laboratory animals
- General principles and requirements
- The health and welfare of animals
- Health and infectious status
- Considerations for Animal Models with Special Needs
- Animal provisions during shipment
- Transport container design and construction
- Transport container stocking density
- Stressors during animal transport
- Journey planning.
- Notes:
- Electronic reproduction. Hoboken, N.J. Available via World Wide Web.
- Description based on online resource; title from digital title page (viewed on March 26, 2024).
- ISBN:
- 9781119555278
- 1119555272
- 9781119555254
- 1119555256
- Publisher Number:
- 90101705615
- Access Restriction:
- Restricted for use by site license.
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