Whole Cell Biocatalysis : Fundamentals and Applications.

Format:

Book

Author/Creator:

Huerta-Ochoa, Sergio.

Contributor:

Prado-Barragan, Arely.

Aguilar, Cristóbal Noé.

Series:

Foundations and Frontiers in Enzymology Series

Language:

English

Physical Description:

1 online resource (422 pages)

Edition:

1st ed.

Place of Publication:

Chantilly : Elsevier Science & Technology, 2025.

Summary:

Whole Cell Biocatalysis, a volume in the Foundations and Frontiers of Enzymology series, offers a detailed overview of the process of biocatalysis using whole cells as an alternative to enzyme biocatalysis.

Contents:

Intro

Whole-Cell Biocatalysis: Fundamentals and Applications

Copyright

Contents

Contributors

About the editors

Preface

Chapter 1: Advantages and new potential applications of whole-cell biocatalysis

1. Introduction

1.1. History of whole-cell biocatalysis development

1.2. Technical advances and economic advantages of whole-cell biocatalysis

1.3. Reaction media in whole-cell biocatalysis

1.4. Main microorganisms used as whole-cell factories

2. Key advances and potential applications

2.1. Cell permeabilization

2.2. Cell immobilization

2.3. Metabolic engineering

2.4. Cascade reactions

2.5. Chemoenzymatic synthesis

2.6. Sustainable manufacturing

2.7. Pharmaceutical production

2.8. Biodegradation and bioremediation

2.9. Renewable energy production

3. Trends and perspectives

References

Chapter 2: Reprogramming microbial cells to improve the production of biopharmaceuticals and fine chemicals

1. Introduction to molecular genetics in the production of chemical and pharmaceutical substances

1.1. Significance of chemical and pharmaceutical substance production in the industry and their impact on the global economy

1.2. Use of microorganisms in the production of chemical and pharmaceutical substances, with emphasis on fungi

1.3. Improving fungal strains through classical genetic techniques with emphasis on antibiotics

1.4. Reasons for the use of molecular genetic techniques

2. Classic molecular cloning techniques

2.1. Molecular cloning: A clear definition

2.2. Cloning of genes and DNA fragments

2.3. DNA and complementary DNA (cDNA) libraries

2.4. Featured examples of molecular cloning in antibiotic production

3. Gene dosage optimization

3.1. Gene dosage and modulation of gene dosage.

3.2. Gene dosage optimization in industrial production: Importance and examples

3.3. Other alternatives: E.g., increasing precursor availability and/or improving precursor and penicillin transport

4. Advanced genetic engineering tools

4.1. Advances in genetic engineering

4.2. High-throughput sequencing (NGS) techniques

4.3. Promoters and RBS (bio-bricks) libraries

4.4. Synthetic biology

4.5. CRISPR-Cas9 technology

5. Cell factories for whole-cell biocatalysis

5.1. Minimal cell factories

5.2. Robust cell factories

5.3. Schemes for autonomous control of the metabolic fluxes and induction of product synthesis

6. The future of molecular genetics in the production of chemical and pharmaceutical substances

Chapter 3: Mitigation of greenhouse gas emissions from biogas-producing facilities: A novel whole-cell technology platfor ...

2. GHG emissions from biogas-producing facilities

3. Conventional aerobic biotechnologies for treating residual dissolved methane

3.1. Aerobic methanotrophic metabolism

3.2. Packed bed reactors and two-phase partitioning systems

3.3. Aerobic membrane bioreactors

4. Whole-cell technology platform for anaerobic methane oxidation

4.1. Fundamentals and process microbiology of the N-AOM process

4.2. Bioreactors and operating conditions reported for N-AOM implementation

5. Perspectives

Chapter 4: Computational metabolic engineering using genome-scale metabolic models and constraint-based methods

1. Defining metabolic engineering

2. Microbial cell factory

3. Strategies for designing microbial cell factories

4. The engineering cycle

5. The principles for the calculation of metabolic fluxes

6. Linear programming for metabolic network modeling

7. Genome-scale mathematical modeling.

8. Reconstruction of the metabolic model

9. Metabolic engineering and systems biology

10. Data integration

11. Metabolic engineering and systems biology strategies

Chapter 5: Whole-cell biocatalysis in nonconventional media

2. Nonconventional media used for biocatalysis

2.1. Whole-cell function in nonconventional media

3. Reaction and transport mechanisms in nonconventional media

3.1. Partitioning bioreactors

3.2. Solid-gas bioreactors

4. Applications of reaction in nonconventional media

5. Conclusions

Chapter 6: Nanostructured magnetic systems in whole-cell biocatalysis

2. Coated magnetic nanoparticles and their properties for catalysis

3. Mechanisms of interactions between cells and magnetic nanoparticles

4. Toxicity of magnetic nanoparticles on microbial cells

5. Application of magnetic nanoparticles in catalysis with bacteria and yeast

6. Surface adhesion fermentation using magnetic nanoparticles: Advantages and disadvantages

7. Scale-up considerations

8. Hyperthermia with magnetic nanoparticles and its possible application

9. Future perspectives

Author contributions

Chapter 7: Filamentous fungi as biopharmaceutical protein factories

1. Protein secretion in filamentous fungi

2. Co- or posttranslational transport from ribosome to ER

3. Folding and polypeptide modifications

4. Golgi complex and O-glycosylation

5. SpitzenkÖrper

6. Biopharmaceutical protein production in filamentous fungi

7. Genetic tools for recombinant protein production in filamentous fungi

8. Concluding remarks

Chapter 8: Proteomic analysis: Application to the study of signal transduction pathways in Penicillium chrysogenum an

1. About Penicillium chrysogenum and Acremonium chrysogenum.

2. Cell signaling

3. Proteomics

3.1. Techniques employed in proteomic analysis

3.1.1. 2D-PAGE and 2D-DIGE

3.1.2. Label-free technique

3.2. Proteomic analysis of cell signaling pathways in P. chrysogenum

3.2.1. Pga1-mediated signaling regulates central metabolic protein expression

3.2.2. Ca2+-mediated cell signaling regulates peroxisome protein abundance and increases penicillin production in P. chry ...

3.2.3. Cell signaling of the polyamines 1-3 diaminopropane and spermidine causes changes in the intracellular proteome an ...

4. Conclusions

Chapter 9: Fungal lipase obtained by surface adhesion fermentation using magnetic chitosan-coated nanoparticles

2. Materials and methods

2.1. Microorganisms and crop development

2.2. Support preparation

2.3. Characterization of the immobilization process of A. niger spores on NPM-Q

2.4. Surface adhesion fermentation (SAF)

2.5. Assay for the determination of lipase activity

3. Results and discussion

3.1. Interaction characterization of A. niger spores and NPM-Q

3.2. Comparison of lipase production by submerged fermentation and surface-attachment fermentation

4. Conclusion

Chapter 10: In vitro plant cultures as a viable biotechnological tool for the biosynthesis of steroidal hormones of cl

1.1. Global prospects in the clinical use and industrial production of hormones

1.1.1. Global economic market income in hormones of clinical relevance

1.1.2. Conventional techniques used for industrial production of hormones

Biotransformation cell reactions

Disadvantages of conventional techniques

1.1.3. Alternative in vitro cell culture techniques through biotechnological tools

1.2. Biosynthesis pathways of steroid structures in plant cells

1.2.1. Secondary metabolism of plants.

1.2.2. Mevalonate and nonmevalonate pathways, biosynthesis of plant-based steroid structures

1.2.3. Phytosterols and brassinosteroids

1.2.4. Clinical interest of hormonal structures derived from bioactive secondary metabolites

1.2.5. Final yields of plants bioactive secondary metabolites in the wild

2. Aim of the chapter

3. Methodology

4. Results

4.1. In vitro plant cell cultures by biotechnological techniques

4.1.1. Mevalonate and nonmevalonate pathways that biosynthesize plant-based steroids

4.1.2. Types of techniques for in vitro plant cell culture

Cell suspension cultures

4.1.3. Final yields of secondary metabolites biosynthesized through in vitro cultures

4.2. Analytical methods applied to secondary metabolites produced by plant cell cultures

4.2.1. Techniques for chemical characterization

Gas chromatography

High-performance liquid chromatography (HPLC)

Mass spectrometry

4.2.2. Isolation and purification processes

Open-column chromatography

Preparative plate chromatography

4.2.3. Progesterone and derivatives

5. Discussion

6. Conclusions

Disclaimer

1Introduction1.1Global prospects in the clinical use and industrial production of hormonesIn the last 20ye

Chapter 11: Whole-cell biocatalysis for large-scale production

2. Design of whole-cell biocatalysts

2.1. The optimization and design of biosynthetic pathways

2.2. Improvement of pathway flux

2.3. Dynamic regulation of enzyme concentrations

2.4. Enhanced urban transportation

3. Biocatalysis of whole cells in biphase media

3.1. Biphasic media-catalyzed lipase

3.2. Reactions facilitated by reductase in aqueous-organic media

3.3. Conclusions

4. Immobilization of whole-cell catalyst

4.1. Strategy for entrapment and encapsulation

4.2. Adhesion technique.

4.3. The covalent coupling method.

Notes:

Description based on publisher supplied metadata and other sources.

ISBN:

9780443239991

0443239991

OCLC:

1515462780