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Apobec Enzymes.
- Format:
- Book
- Author/Creator:
- Papavasiliou, Nina.
- Series:
- Methods in Enzymology Series
- Methods in Enzymology Series ; v.Volume 713
- Language:
- English
- Subjects (All):
- RNA editing.
- Mutagenesis.
- Physical Description:
- 1 online resource (312 pages)
- Edition:
- 1st ed.
- Place of Publication:
- Chantilly : Elsevier Science & Technology, 2025.
- Summary:
- This book provides a comprehensive exploration of RNA editing, focusing on methodologies, techniques, and assays related to APOBEC enzymes. It includes detailed protocols for fluorescent shift assays, RNA sequencing, purification of APOBEC proteins, and in vitro deamination assays. The book also discusses mutation signatures, genome-wide mutagenic impacts, and profiling rare RNA editing events. Designed for researchers and professionals in biochemistry and molecular biology, the work emphasizes practical applications and aims to advance understanding of genetic editing mechanisms. Generated by AI.
- Contents:
- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- Preface
- Chapter One: Fluorescent shift assay for APOBEC-mediated RNA editing
- Abstract
- 1 Introduction
- 2 Key materials
- 3 Protocol
- 4 Discussion and conclusion
- 5 Notes
- Acknowledgements
- References
- Chapter Two: Low-error RNA sequencing techniques for detecting RNA editing by APOBECs: Circular RNAseq assay and safe-sequencing system (SSS)
- 2 Circular-sequencing assay
- 3 Materials and reagents
- 4 Methods
- 5 Circular RNAseq assay
- 6 Materials and reagents
- 7 Methods
- 8 Conclusions
- Chapter Three: Purification of enzymatically active APOBEC proteins from an insect cell expression system
- Abstract Generated by AI.
- Notes:
- Description based on publisher supplied metadata and other sources.
- Part of the metadata in this record was created by AI, based on the text of the resource.
- ISBN:
- 0-443-31787-9
- 0-443-31786-0
- OCLC:
- 1518297161
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