Base Editors : Methods and Protocols / edited by Sangsu Bae, Beomjong Song.

Format:

Book

Contributor:

Bae, Sangsu., Editor.

Song, Beomjong., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2606

Language:

English

Subjects (All):

Biology--Technique.

Biology.

Genetics.

Genetic Techniques.

Local Subjects:

Genetic Techniques.

Genetics.

Physical Description:

1 online resource (XII, 235 p. 48 illus., 36 illus. in color.)

Edition:

1st ed. 2023.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

Summary:

This volume explores base editors (BEs), an invaluable CRISPR-based genome editing tool with a wide variety of versatile applications. Beginning with an overview of BEs, their diverse variants, and computational tools, the book continues with experimental applications of BEs for disease modeling in mammalian cells and generating mutagenic mice, therapeutic base editing strategies, which covers delivery methods of BE-encoded DNA plasmids, mRNAs, or ribonucleoproteins through viruses or non-viral lipid nanoparticles, and lastly, the use of BEs in plants and bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Base Editors: Methods and Protocols serves as an ideal guide for researchers looking to use base editors to continue their studies in an array of fields.

Contents:

Introduction and Perspectives of DNA Base Editors

Web-Based Computational Tools for Base Editors

Prediction of Base Editing Efficiencies and Outcomes Using DeepABE and DeepCBE

Profiling Genome-Wide Specificity of dCpf1 Cytidine Base Editors Using Digenome-Seq

Base Editing of Human Hematopoietic Stem Cells

A/C Simultaneous Conversion Using the Dual Base Editor in Human Cells

Functional Analysis of Variants in BRCA1 Using CRISPR Base-Editors

Use of the Representative Base Editing Tool Target-AID to Introduce Pathogenic Mutations into Mice

Targeted Mutagenesis in Mice Using a Base Editor

Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor

Delivering Base Editors In Vivo by Adeno-Associated Virus Vectors

The Delivery of ABE mRNA to the Adult Murine Liver by Lipid Nanoparticles (LNPs)

Ex Vivo Base Editing Therapy with Chemically-Derived Hepatic Progenitors

Application of Base Editor-Mediated Genome Editing in Mouse Retina.-High-Throughput Base-Editing-Mediated Artificial Evolution Streamlines Trait Gene Identification in Rice

Heritable Virus-Induced Genome Editing (VIGE) in Nicotiana attenuate

Cytosine Base Editing in Bacteria.

ISBN:

9781071628799

1071628798