The Tumor Microenvironment : Methods and Protocols / edited by Josie Ursini-Siegel.

Format:

Book

Contributor:

Ursini-Siegel, Josie., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2614

Language:

English

Subjects (All):

Cancer.

Cancer Microenvironment.

Local Subjects:

Cancer Microenvironment.

Physical Description:

1 online resource (XIV, 414 p. 91 illus., 76 illus. in color.)

Edition:

2nd ed. 2023.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

Summary:

This second edition provides update and new chapters detailing core and emerging in vitro and in vivo protocols. Chapters guide readers through cellular and molecular biology approaches, in vivo genetic approaches, various “omics”-based strategies, therapeutic strategies, and advanced techniques in the fields of tissue engineering and nanotechnology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, The Tumor Microenvironment: Methods and Protocols, Second Edition is a valuable resource for both novice and expert scientists in this developing field.

Contents:

Imaging Mass Cytometry in Immuno-oncology

Analyzing the Tumor Immune Microenvironment by Flow Cytometry

Blood sample processing and banking for functional and molecular analyses

Quantitatively Assessing the Respiratory Burst in Innate Immune Cells

Visualizing NETosis using a Novel Neutrophil Extracellular Trap-Specific Marker

In vitro methods to evaluate macrophage polarization and function in cancer

In vivo analysis of tumor associated macrophages in the tumor microenvironment

Assessing the immune regulatory role of metabolites in vitro

Mouse Models of Obesity To Study The Tumor Immune Microenvironment

Assessing the efficacy of immune checkpoint inhibitors in pre-clinical tumor models

Fibroblast isolation from mammary gland tissue and syngeneic murine breast cancer models

Multiscale label-free imaging of fibrillar collagen in the tumor microenvironment

In situ measurement of intra-tumoral tissue rigidity

Extracellular matrix glycation and crosslinking in mammary tumor progression

Proteomic assessment of the murine mammary gland extracellular matrix

Spheroids as a 3D Model of the Hypoxic Tumor Microenvironment

Tumor pH imaging using Chemical Exchange Saturation Transfer (CEST)-MRI

Profiling the epigenetic landscape of the tumor microenvironment using chromatin immunoprecipitation-sequencing

Analysis of Spatial Molecular Data

Laser-assisted single-cell labeling and capture

3D hydrogel cultures for high-throughput drug discovery

Intravital microscopy of the metastatic pulmonary environment

A CRISPR platform for targeted in vivo screens. .

ISBN:

9781071629147

107162914X