Clinical Applications of Nucleic Acid Amplification / edited by Meagan B. Myers, Cynthia A. Schandl.

Format:

Book

Contributor:

Myers, Meagan B., Editor.

Schandl, Cynthia A., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2621

Language:

English

Subjects (All):

Biomaterials.

Nucleic acids.

Biochemistry.

Nucleic Acid.

Chemical Biology.

Local Subjects:

Nucleic Acid.

Biochemistry.

Chemical Biology.

Physical Description:

1 online resource (XIV, 345 p. 84 illus., 67 illus. in color.)

Edition:

1st ed. 2023.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

Summary:

This volume details applications, methods, and protocols on nucleic acid amplification. Chapters detail the management of organ and stem cell transplantation, constitutional genetics and genomics, epidemiology, public health, nucleic acid detection, methods for amplification and sequencing, nuclease-assisted mutant allele enrichment, isothermal, and high-throughput sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Clinical Applications of Nucleic Acid Amplification is a valuable resource for both novice and expert scientists in this developing field.

Contents:

A Brief Practical Guide to PCR

Managing a PCR Contamination Event in a Molecular Pathology Laboratory

Next Generation Sequencing Informatic Architecture Considerations

Nuclease Enrichment and qPCR Detection of Rare Nucleotide Variants

MYD88 L265P Mutation Detection by ddPCR: Recommendations for Screening and Minimal Residual Disease Monitoring

Capture Methylation-Sensitive Restriction Enzyme Sequencing (Capture MRE-Seq) for Methylation Analysis of Highly Degraded DNA

LT-RPA: An Isothermal DNA Amplification Approach for Improved Microsatellite Genotyping and Microsatellite Instability Detection

Liquid Biopsy for Advanced Cancer: An Amplicon Based Massively Parallel Sequencing Panel Approach to Precision Oncology

Mate Pair Sequencing: Next Generation Sequencing for Structural Variant Detection

Cell Subset-Specific Chimerism Testing by Short Tandem Repeats Analysis for Engraftment Monitoring After Hematopoietic Stem Cell Transplantation

Immune Cell Lineage-Specific Chimerism Testing by Next-Generation Sequencing for Engraftment Monitoring After Allogeneic Hematopoietic Stem Cell Transplantation

Rapid Whole Genome Sequencing for Diagnosis of Single Locus Genetic Diseases in Critically Ill Children

EOSAL-CNV for Easy and Rapid Detection of CNVs by Fragment Analysis

Rapid Cycle and Extreme Polymerase Chain Reaction

CRISPR-Cas12-Based Diagnostic Applications in Infectious and Zoonotic Diseases

SARS-CoV-2 Sequencing for Variant Surveillance

Tuberculosis Diagnosis Using Isothermal Nucleic Acid Amplification in a Paper-and-Plastic Device

Loop-Mediated Isothermal Amplification on Paper Microfluidic Chips for Highly Sensitive and Specific Zika Virus Detection Using Smartphone

Ultrasensitive Reverse Transcriptase Loop-Mediated Isothermal Amplification (US-LAMP) Based Detection of Malaria Infection from Dried Blood Spots. .

ISBN:

9781071629505

1071629506