Serum/Plasma Proteomics : Methods and Protocols / edited by David W. Greening, Richard J. Simpson.

Format:

Book

Contributor:

Greening, David W., Editor.

Simpson, Richard J., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2628

Language:

English

Subjects (All):

Proteins.

Bioinformatics.

Mass spectrometry.

Mass Spectrometry.

Local Subjects:

Proteins.

Bioinformatics.

Mass Spectrometry.

Physical Description:

1 online resource (XVIII, 596 p. 60 illus., 50 illus. in color.)

Edition:

3rd ed. 2023.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

Summary:

This third volume provides comprehensive protocols on pre-analytical, analytical, plasma, and serum proteomics. New and updated chapters are divided into nine sections, detailing blood processing and handling strategies, discovery- and targeted-based mass spectrometry, including workflows to aid in discovery and targeted data analysis, in addition to software and bioinformatics for the plasma proteome. This edition further integrates emerging areas in the development of technologies for plasma proteomics and assay platforms in biomarker discovery and translational proteomics, enrichment and detection strategies to understand the plasma proteome, and peptide, lipid and metabolite targeted assays. We also detail the emerging analysis of extracellular vesicles isolated from plasma. Written in the format of the highly successful Methods in Molecular Biology series, each of the 33 chapters includes an introduction to the topic, lists necessary materials and methods, includes hints and tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Serum/Plasma Proteomics: Methods and Protocols, Third Edition aims to be comprehensive guide for researchers. .

Contents:

Protocols for the Isolation of Platelets for Research and Contrast to Production of Platelet Concentrates for Transfusion

Collection of plasma samples in areas with limited healthcare access

Blood Collection Processing & Handling for Plasma and Serum Proteomics

Preparation of cryoprecipitate and cryo-depleted plasma for proteomic research analysis

High throughput proteome profiling of plasma and native plasma complexes using native chromatography

High-throughput and in-depth proteomic profiling of 5 µL plasma and serum using TMTpro 16plex

In depth data-independent acquisition proteomics of human plasma identifies low abundant proteins

In-depth Blood Proteome Profiling by Extensive Fractionation and multiplexed quantitative mass spectrometry

Early cancer biomarker discovery using DIA-MS proteomic analysis of EVs from peripheral blood

Strategies to enrich, identify and characterize glycoproteome in blood plasma using Liquid Chromatography High Resolution Mass Spectrometry

Proteome analysis of whole blood collected by volumetric absorptive microsampling

Proteomic applications and considerations: from research to patient care

Immunoaffinity mass spectrometry diagnostic tests for multi-biomarker assays

Secretome profile of leukocyte-platelet rich fibrin (L-PRF) membranes

Quantification of proteins in blood by absorptive microtiter plate-based affinity purification coupled to liquid chromatography-mass spectrometry

Glycomics-assisted Glycoproteomics Enables Deep and Unbiased N-Glycoproteome Profiling of Complex Biological Specimens. -The Small Protein Enrichment Assay (SPEA) for analysis of low abundance peptide hormones in plasma

In-depth proteomic analysis of blood circulating small extracellular vesicles

Protocol for Plasma Extracellular Vesicle and Particle Isolation and Mass Spectrometry-based Proteomic Identification

Analysis of ExtracellularVesicle and Contaminant Markers in Blood Derivatives Using Multiple Reaction Monitoring

Generation of red blood cell nanovesicles as a delivery tool

Progress in targeted mass spectrometry (parallel accumulation-serial fragmentation) and its application in plasma/serum proteomics

Offline peptide fractionation and parallel reaction monitoring MS for the quantitation of low-abundance plasma proteins

Profiling Serum Intact N-Glycopeptides Using Data-Independent Acquisition Mass Spectrometry

Semi-automated lectin magnetic bead array (LeMBA) for translational serum glycoprotein biomarker discovery and validation

Accessing antibody-reactivities in serum or plasma to (auto-)antigens using multiplexed bead-based protein immunoassays

Absolute quantitative targeted proteomics assays for plasma proteins

Rapid and quantitative enrichment of peptides from plasma for mass spectrometric analysis

Comprehensive targeted lipidomic profilingfor research and clinical applications

Multiplexed bead-based peptide immunoassays for the detection of antibody reactivities

Array-based multiplex and high-throughput serology assays

Bioinformatic tools and knowledgebases to assist generating targeted assays for plasma proteomics

Use of Longitudinal Serum Analysis and Machine Learning to Develop a Classifier for Cancer Early Detection.

ISBN:

9781071629789

1071629786