MicroRNA Detection and Target Identification : Methods and Protocols / edited by Tamas Dalmay.

Format:

Book

Contributor:

Dalmay, Tamas., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2630

Language:

English

Subjects (All):

Genetics.

RNA--Metabolism.

RNA.

Genetics and Genomics.

RNA Metabolism.

Local Subjects:

Genetics and Genomics.

RNA Metabolism.

Physical Description:

1 online resource (X, 266 p. 41 illus., 36 illus. in color.)

Edition:

2nd ed. 2023.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

Summary:

This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aimsto ensure successful results in the further study of this vital field.

Contents:

Detection of miRNAs

MicroRNA Identification, Target Prediction, and Validation for Crop Improvement

MicroRNA Detection with CRISPR/Cas

Detection of MicroRNAs by Northern Blot

MicroRNA Detection at Femtomolar Concentrations with Isothermal Amplification and a Biological Nanopore

Detection of MicroRNAs Expression Dynamics Using LNA/DNA Nanobiosensor

Programmable Ultrasensitive Molecular Amplifier for Digital and Multiplex MicroRNA Quantification

Small RNA Profiling by Next Generation Sequencing Using High-Definition Adapters

Quantification of MicroRNAs or Viral RNAs with Microelectrode Sensors Enabled by Electrochemical Signal Amplification

Discovery and Evaluation of Extracellular MicroRNA Biomarkers in Plasma, Ascites, and Urine

Determining miRNA Expression Patterns in Xenopus

Overview of Computational and Experimental Methods to Identify Tissue-Specific MicroRNA Targets

sRNAtoolbox: Dockerized Analysis of Small RNA Sequencing Data in Model and Non-Model Species

MicroRNA-Target Identification: A Combinatorial In Silico Approach

An Efficient CRISPR-Cas9 Method to Knockout miRNA Expression in Xenopus tropicalis

Interrogation of Functional miRNA-Target Interactions by CRISPR/Cas9 Genome Engineering.

ISBN:

9781071629826

1071629824