Astrocytes : Methods and Protocols / edited by Barbara Di Benedetto.

Format:

Book

Contributor:

Di Benedetto, Barbara, Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2896

Language:

English

Subjects (All):

Neuroanatomy.

Neurosciences.

Nervous System.

Neuroscience.

Local Subjects:

Nervous System.

Neuroscience.

Physical Description:

1 online resource (XI, 288 p. 53 illus., 51 illus. in color.)

Edition:

2nd ed. 2025.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2025.

Summary:

This detailed volume gathers methods essential for investigating the multifaceted roles of astrocytes in both physiology and pathological conditions. The book covers several techniques for the preparation of astrocytes from animal models, the examination of astrocyte morphology and functions in vitro and ex vivo, as well as the investigation of astrocyte pathologies in the central nervous system. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Astrocytes: Methods and Protocols, Second Edition serves as an ideal guide for both experienced and beginner scientists working toward unravelling the roles of these highly versatile cells.

Contents:

Immunofluorescence Protocol to Label Astrocytes from Diverse Model and Non-Model Mammals

Retro-Orbital Delivery of AAVs for CNS-Wide Astrocyte Targeting

An Empirical-Theoretical Approach to Determine Astroglial Potassium upon Ischemic Stress

Quantification of Astrocytic Sodium Signals Using Fluorescence Lifetime Imaging (FLIM)

pH Sensor-Based Engulfment Assays to Measure Phagocytic Activity of Astrocytes

An Astrocyte Transplantation Method to Investigate Astrocyte Development and Diversity in the CNS

Primary CNS Cultures with Mixed Neural Cell Types to Study Correlative Effects of High Glucocorticoids on Astrocytes, Oligodendrocytes, and Myelination Markers

Postnatal Development of Perivascular Astrocytic Processes and Detection of Local mRNA and Translation

Imaging Techniques for the Analysis of the Blood-Brain Barrier and Gliovascular Unit Dysfunction

Assessment of Blood-Brain Barrier Integrity Using Dyes and Tracers

Investigating the Pathology-Related Astroglial Plasticity in the Human Cerebral Cortex

Super-Resolution Analysis of Astrocyte Morphology Using Expansion Microscopy

Astrocyte Morphological Adaptations in a Mouse Model of Social Anxiety Disorder

An In Vitro Assay to Measure Astrocyte-Dependent Synaptic Phagocytosis in Health and Major Depressive Disorder

Cortical Organotypic Brain Slice Cultures to Examine Sex- and Age-Dependent Astrocyte-Mediated Synaptic Phagocytosis

Development and Validation of Stress Paradigms to Study Astrocytes in Rodent Models of Stress Disorders

Cellular Investigation of Astrocytic Neuroendocrine Responses to Stress

Morphological Investigation of Astrocytic Responses to Stress

Functional Evaluation of Astrocytic Responses to Stress

Unveiling Ketamine's Influence on Astrocytic Kir4.1 Channels through Multi-Modal Analysis: Confocal Microscopy, Immunocytochemistry, Fluorescence Analysis, and Electrophysiology

Revealing and Characterizing Astrocytic Atrophy in an Animal Model of Depression.

ISBN:

9781071643662

1071643665