CRISPR-Cas systems for RNA and genome editing. Part A. / edited by Ailong Ke and Weixin Tang.

Format:

Book

Contributor:

Ke, Ailong, editor.

Tang, Weixin, editor.

Series:

Methods in enzymology ; Volume 712.

Methods in Enzymology Series ; Volume 712

Language:

English

Subjects (All):

CRISPR-associated protein 9.

Gene editing.

Physical Description:

1 online resource (513 pages)

Edition:

First edition.

Place of Publication:

Cambridge, MA : Academic Press, [2025]

Summary:

CRISPR-Cas Systems for RNA and Genome Editing, Volume 711 in this ongoing series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites, Genome oligopaint via local denaturation fluorescence in situ hybridization, Biochemical reconstitution of a type I-B CRISPR-associated transposon, Visualizing the conformational landscape of CRISPR-Cas9 through kinetics-informed structural studies, and much more.Additional sections cover Analysis of metal-dependent DNA nicking activities by Cas endonucleases, Biochemically characterizing the accessory proteins and ribonucleoprotein complexes of Type IV CRISPR systems, Prime editing in bacteria with BacPE, Preparation of high-purity CRISPR-based DNA base editor RNPs, Bacterial directed evolution of CRISPR base editors, Cloning and validating systems for high throughput molecular recording, Using CRISPR-Cas13 for viral nucleic acid detection, and more.- Provides the latest information on DNA/RNA research- Offers outstanding and original reviews on a range of DNA/RNA research topics- Serves as an indispensable reference for researchers and students alike

Notes:

Includes bibliographical references.

Description based on publisher supplied metadata and other sources.

Description based on print version record.

ISBN:

9780443317538

0443317534

9780443317521

0443317526

OCLC:

1512320535