Nutrient Sensing in Eukaryotes / edited by Fiona M. Russell, D. Grahame Hardie.

Format:

Book

Contributor:

Russell, Fiona M., Editor.

Hardie, D. Grahame., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2882

Language:

English

Subjects (All):

Biochemistry.

Cytology.

Cell Biology.

Local Subjects:

Biochemistry.

Cell Biology.

Physical Description:

1 online resource (XIII, 330 p. 48 illus., 47 illus. in color.)

Edition:

1st ed. 2025.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2025.

Summary:

This detailed volume explores various methods through which nutrient sensing can be studied, particularly in multicellular eukaryotes. Beginning with a section on the monitoring of cellular energy status by sensing of adenine nucleotides, the book continues by delving into numerous techniques for researching both intracellular and extracellular nutrient sensing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nutrient Sensing in Eukaryotes serves as an ideal guide for researchers aiming to develop related or novel methods to study the sensing of nutrients in their own systems. Chapter 3 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Contents:

Measuring Cellular Adenine Nucleotides by Liquid Chromatography Coupled Mass Spectrometry

FRET-Based Sensor for Measuring Adenine Nucleotide Binding to AMPK

Monitoring Cellular Energy Balance in Single Cells Using Fluorescent Biosensors for AMPK

Assays of Different Mechanisms of AMPK Activation, and Use of Cells Expressing Mutant AMPK to Delineate Activation Mechanisms

Methods to Determine Lysosomal AMPK Activation

Sensing of Long Chain Fatty Acyl-CoA Esters by AMPK

Visualization of Subcellular mTOR Complex 1 Activity with a FRET-Based Sensor (TORCAR)

Determination of Nutrient Ligand - Sensor Binding Affinity

Amino Acid Sensing by Transceptors: Exploring Substrate-Induced Regulation of Amino Acid Transporters and Transporter Expression

Purification and Analysis of eIF2α Phosphorylation by Stress-Activated Protein Kinase Gcn2 from S. cerevisiae

An In Vitro System to Study Mammalian GCN2 Regulation

Sensing of Cholesterol by Squalene Monooxygenase

Large Scale Protein Production and Activity Assay Protocols for Human Glycogen Synthase-Glycogenin Complex

Measurement of Neurotransmitters and Metabolites in Cell Culture and In Vitro Using Genetically Encoded PBP-Based Biosensors

Calcium Imaging in Hypothalamic Cells

Nutrient Sensing by Lingual G-Protein Coupled Taste Receptors.

ISBN:

9781071642849

1071642847