Flow Cytometry : Principles, Applications, and Clinical Perspectives / edited by Ranbir Chander Sobti, Awtar Krishan, Devendra K. Agrawal.

Format:

Book

Author/Creator:

Sobti, R. C.

Contributor:

Krishan, Awtar.

Agrawal, Devendra K.

Series:

Biomedical and Life Sciences Series

Language:

English

Subjects (All):

Biology--Technique.

Biology.

Imaging systems in biology.

Analytical biochemistry.

Biological Techniques.

Biological Imaging.

Analytical Biochemistry.

Local Subjects:

Biological Techniques.

Biological Imaging.

Analytical Biochemistry.

Physical Description:

1 online resource (483 pages)

Edition:

1st ed. 2024.

Place of Publication:

Singapore : Springer Nature Singapore : Imprint: Springer, 2024.

Summary:

Flow cytometry is a state-of-the-art technology that is widely used in biological research and clinical diagnostics. It is a high-throughput platform that allows the quantification, differentiation, and functional study of cells or cell like particles in suspension. Various cell components can be targeted with fluorescently labelled antibodies or fluorescent dyes to allow measurement and analysis of the physical, chemical, and biological properties of individual cells within homogenous or heterogeneous populations. Since its commercialization more than five decades ago, flow cytometry has advanced and become an very important analytical tool in translational research. With the advancement of instrument technology, an increasing number of fluorescent dyes, and an expanding range of monoclonal antibodies, the applications of flow cytometry in applied research continue to grow. The use of multiparametric flow cytometry in translational research provides the ability to rapidly identify different cell populations and to simultaneously measure multiple parameters of single cells for efficiently assessing immune status, decrease/increase of specific immune cell populations, cell activation status, etc. of different cells in preclinical and clinical studies. The primary goal of any research work is to take research results from the "bench to-bed and back". The use of flow cytometry in applied research advances the development of new diagnostic tests or drugs for cancer treatment, immune monitoring, etc. that help in patient care. Today, every biological scientist needs to have basic knowledge of flow cytometry in order to utilise this technology properly in their own research and to understand other’s research work. The present book has been designed to give the knowledge of flow cytometry and its applications to the researchers and teachers. It will allow the readers to utilize the technology in an appropriate way in their research work. This book has describing various applications of flow cytometry like cell health monitoring, immunophenotyping, cell sorting, stem cell characterization, micro-vesicle analysis etc.

Contents:

Chapter 1. Flow cytometry: historical perspectives, fundamentals, past and present instrumentations and applications

Section 1. Instrumentation

Chapter 2. Instrumental and experimental quality control in flow cytometry

Chapter 3. Redox landscape in flow cytometry

Section 2. Applications in basic sciences

Chapter 4. Unravelling biological complexities through flow cytometric applications

Chapter 5. Illuminating cell dynamics: the versatility of flow cytometry in molecular biology analysis

Chapter 6. Instrumental role of flow cytometry in molecular biology advancements

Chapter 7. Flow cytometry: revolutionizing cellular analysis in spectral and microfluidic dimensions

Chapter 8. Immunological kaleidoscope: flow cytometry's colorful insight into cells

Chapter 9. Flow cytometric immunophenotyping of leukocytes and neutrophils subsets in bone marrow, peripheral blood and liver tissue

Chapter 10. Flow cytometric analysis of plant nuclear DNA content in plant homogenates

Chapter 11. Flow cytometry in microbiological sciences

Section 3. Applications in clinical and transplantation sciences

Chapter 12. Applications of flow cytometry in scientific research and the clinic

Chapter 13. Flow cytometric monitoring of the redox status in health and disease

Chapter 14. multi-color flow cytometry in medical research: a versatile tool to evaluate the rare immune cells in the pathogenesis of chronic viral infections

Chapter 15. Flow cytometry approach to differentiate resident versus infiltrated macrophages in atherosclerosis

Chapter 16. Cytokine analysis in leukemia and minimal residual disease (mrd) detection through flow cytometry

Chapter 17. Flow cytometry in transplantation

Chapter 18. Applications of flow cytometry in transplant medicine

Chapter 19. Flow cytometry based residual disease monitoring in haematolymphoid neoplasm

Chapter 20. Clinical significance of flow cytometry in solid organ transplantation

Section 4. Translational, stem cells and regenerative studies

Chapter 21. An introduction to multiparametric flow cytometry in translational research

Chapter 22. Stem cels and micro vesicles analysis

Chapter 23. Translational flow cytometry for repopulating cells in regenerative medicine

Section 5

Chapter 24. Flow cytometry in drug design and delivery

Chapter 25. Flow cytometry in toxicology:illuminating cellular responses

Section 6. Flow cytometry and environmental studies

Chapter 26. Exploring the role and potential of flow cytometry in advancing environmental research

Chapter 27. Role of flow cytometry in the analysis of micro/nano plastics.

Notes:

Description based on publisher supplied metadata and other sources.

Other Format:

Print version: Sobti, Ranbir Chander Flow Cytometry

ISBN:

9789819745531

OCLC:

1482237407