Molecular Motors : Methods and Protocols / edited by Christophe Lavelle, Antoine Le Gall.

Format:

Book

Contributor:

Lavelle, Christophe, Editor.

Le Gall, Antoine., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2881

Language:

English

Subjects (All):

Cytology.

Cell Biology.

Local Subjects:

Cell Biology.

Physical Description:

1 online resource (XII, 358 p. 73 illus., 65 illus. in color.)

Edition:

3rd ed. 2025.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2025.

Summary:

This fully updated volume presents a diverse collection of protocols and methodologies for studying various molecular motors. The book explores the dynamics of the bacterial flagellar motor, the mechanisms of ATP synthase, the assembly and function of cytoskeletal motors, and the role of nucleic acid motors in cellular processes, while placing a strong emphasis on state-of-the-art techniques, highlighting innovative methods such as single-molecule visualization and high-resolution tracking, sophisticated biochemical assays, bioengineering applications, as well as computational modeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Molecular Motors: Methods and Protocols, Third Edition serves as an ideal guide to enable researchers to probe the mechanisms of molecular motors with unprecedented precision and detail.

Contents:

Cellular and Nuclear Forces: An Overview

Measuring Bacterial Flagellar Motor Dynamics via a Bead Assay

Purification and Reconstitution of Ilyobacter tartaricus ATP Synthase

Motor Function of the Two-Component EEA1-Rab5 Revealed by dcFCCS

Tracking Single Kinesin in Live Cells Using MINFLUX

Multicolor Tracking of Molecular Motors at Nanometer Resolution

Engineering Dynein Motors to Move on DNA Nanotube Tracks

Engineering Synthetic Myosin Filaments Using DNA Nanotubes

Expression, Purification, and In Vitro Analysis of Myosin

Single-Molecule Visualization of BLM-DNA2-Mediated DNA End Resection Using DNA Curtains

Isolation and Analysis of Rare Enzymatic Events with Multiplex Flow Magnetic Tweezers

Homologous Recombination and DNA Intermediates Analyzed by Electron Microscopy

Electrophoretic Analysis of DNA Supercoiling Activities

Remodeling of Individual Nucleosomes in Nucleosome Arrays

Methods for Studying Motor-Driven Viral DNA Packaging in Bacteriophages phi29, Lambda, and T4 via Single DNA Molecule Manipulation and Rapid Solution Exchange

Preparation of a Substrate for Studying Nucleosomes on Single Molecules of DNA

Assessing the Effect of Chromatin-Binding Enzymes on Condensed Chromatin with Optical Tweezers.

ISBN:

9781071642801

1071642804