Vaccinia, Mpox, and Other Poxviruses : Methods and Protocols / edited by Zhilong Yang, Panayampalli Subbian Satheshkumar.

Format:

Book

Contributor:

Yang, Zhilong, Editor.

Satheshkumar, Panayampalli Subbian., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2860

Language:

English

Subjects (All):

Virology.

Local Subjects:

Virology.

Physical Description:

1 online resource (XIV, 362 p. 67 illus., 58 illus. in color.)

Edition:

1st ed. 2025.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2025.

Summary:

This detailed volume covers new and classic methods for vaccinia virus (the prototype poxvirus) but also extends to methods and protocols specifically tailored for mpox virus, lumpy skin disease virus, and myxoma virus. As these viruses continue to expand their territories, research into the impact of poxviruses on human and animal health, as well as investigating vaccine vectors and oncolytic virotherapy, becomes more important, and this book provides a thorough resource for this area of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Vaccinia, Mpox, and Other Poxviruses: Methods and Protocols serves as an ideal resource for virologists working in this vital section of the field.

Contents:

Poxvirus Epidemiology

Mpox Specimen Collection, Storage, Preparation, and Laboratory Diagnostics by PCR

A Quantitative Real-Time PCR Assay for Measuring Poxvirus Replication and Cell Binding

Orthopoxvirus Genome Sequencing, Assembly, and Analysis

Bioinformatics for the Structural Genomics of Poxviruses

Construction and Isolation of Recombinant Vaccinia Virus by Homologous Recombination Using Fluorescent Protein Markers

Rapid Generation of Recombinant Poxviruses Using CRISPR/Cas9 Gene Editing

Cas9-Mediated Poxvirus Recombinant Recovery (CASPRR) for Fast Recovery of Recombinant Vaccinia Viruses

Use of a Poxvirus K3 Ortholog as a Positive Selection Marker for Construction of Recombinant Vaccinia Virus with Modified Host Range

Orthopoxvirus Purification for High Containment Laboratories

Evaluation of Vaccinia Virus Infection in Mice Using Two-Reporter Recombinant Virus

Bioluminescence Imaging to Study Recombinant Orthopoxvirus Infection in Animal Models

Methods to Study Poxvirus Structures by Cryo-EM Imaging Modalities

Investigating the Impact of Host Factors on Vaccinia Virus Infection through Single-Cell Analysis via Flow Cytometry

High-Throughput Single Cell Analysis of Vaccinia Virus Infection

Experimental Evolution of Poxviruses

An Evolutionary Framework Exploiting Virologs and Their Host Origins to Inform Poxvirus Protein Functions

Sample Preparation for Global Metabolic Profiling of Vaccinia Virus-Infected Primary Human Foreskin Fibroblasts

Modified Vaccinia Virus Ankara Amplification and Titration Using Crystal Violet- or Immuno-Staining in DF-1 Cells

Generating MVA-Vector Vaccine Candidates and Testing Them in Animal Models

Isolation and Identification of Lumpy Skin Disease Virus

Testing Oncolytic Myxoma Virus in Immunocompetent Mouse Model for Cancer Therapy.

ISBN:

9781071641606