Intracellular Lipid Transport : Methods and Protocols / edited by Guillaume Drin.

Format:

Book

Contributor:

Drin, Guillaume., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2888

Language:

English

Subjects (All):

Cytology.

Lipids.

Microbiology.

Cell Biology.

Lipidology.

Cellular Microbiology.

Local Subjects:

Cell Biology.

Lipidology.

Cellular Microbiology.

Physical Description:

1 online resource (XI, 325 p. 81 illus., 61 illus. in color.)

Edition:

2nd ed. 2025.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2025.

Summary:

This fully updated volume explores different classes of lipid transporters, such as lipid transfer proteins, flippases, and scramblases, and significant advances in solving their biological functions. The first half of the book features methodologies to measure the movement of lipids between or in organelle membranes inside eukaryotic cells, including plant cells, or in prokaryotic cells, and the book continues with in vitro or in silico approaches aiming to define, more from a biochemical and structural standpoint, how lipid transfer proteins function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Intracellular Lipid Transport: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to shed light on diverse aspects of this critical and often elusive cellular process.

Contents:

Selective Labeling of Phosphatidylcholine to Track Their Translocation Between Organelles in Living Cells

Measurement of ORP10-Mediated Lipid Countertransport at ER-Endosome Membrane Contact Sites Via a Chemically-Induced Dimerization Strategy

Analysis of Phosphatidylinositol Transfer at ER-PM Contact Sites in Receptor-Stimulated Live Cells

Use of the D4H Probe to Track Sterols in Yeast

Maistero-2, a Novel Probe for Sterols: Application for Visualizing Cellular Cholesterol

Using GRAM Domain-Based Biosensors to Monitor Accessible Cholesterol Distribution in Live Cells

Monitoring Accessible Cholesterol Levels in Immune Cells

Methods for Visualizing and Quantifying Cholesterol Distribution in Mammalian Cells Using Filipin and D4 Probes

HEK293 Cell-Based Assay to Measure the Lipid Scrambling Activity of TMEM16 Family Members

Measuring Phospholipid Flippase Activity by NBD-Lipid Uptake in Living Yeast Cells

Advanced Method for the In Vivo Measurements of Lysophospholipid Translocation Across the Inner (Cytoplasmic) Membrane of Escherichia coli

Measurement of Lipid Transport in Mitochondria by the MTL Complex

Determining ATG2 Localization During Autophagosome Formation in Mammalian Cells

Use of Bicelle-Generated Lipid Bilayer Vesicle Nanoparticles for In Vitro Measurement of Lipid Intermembrane Transport

DNA-Assisted Assays for Studying Lipid Transfer Between Membranes

Galactocerebroside Lipid Nanotubes, a Model Membrane System for Studying Membrane-Associated Proteins on a Molecular Scale

In Vitro Approaches for Detection of Protein-Lipid Interactions and Protein-to-Protein Transport of Lipids Using Bifunctional Lipid Analogs

Determining the Relative Affinity of ORPs for Lipid Ligands Using Fluorescence and Thermal Shift Assays

Atomistic Simulations and Analysis of Peripheral Membrane Proteins with Model Lipid Bilayers

In Silico Strategies for Characterizing Inner Cavities of Lipid-Binding Proteins.

ISBN:

9781071643181

1071643185