Bunyaviruses : Methods and Protocols / edited by Hani Boshra.

Format:

Book

Contributor:

Boshra, Hani., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2893

Language:

English

Subjects (All):

Virology.

Local Subjects:

Virology.

Physical Description:

1 online resource (XI, 301 p. 48 illus., 36 illus. in color.)

Edition:

1st ed. 2025.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2025.

Summary:

This volume explores the latest advancements in the study of bunyaviruses. The chapters in this book cover topics such as virus manipulation and purification; expression of recombinant proteins; the experimental study of bunyavirus infection in animals for virological and immunological analysis; and plant bunyaviruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble shooting and avoiding known pitfalls. Comprehensive and cutting-edge, Bunyaviruses: Methods and Protocols is a valuable resource for both expert and novice scientists who are interested in learning more about this important and developing field.

Contents:

An Introduction to the Class Bunyaviricetes

Use of Rift Valley Fever Virus Expressing NanoLuc Luciferase for the Assessment of Neutralizing Antibodies and Antivirals

A Serological Assay for the Detection of Antibodies Generated Against California Serogroup Virus Infection

Molecular Diagnosis of Phlebovirus riftense Infection Using an L-Segment Based Real-Time RT-PCR Method

Ultracentrifugation Purification of Cache Valley Virus Using Iodixanol

Cryogenic Electron Microscopy of Rift Valley Fever Virus

Detection and Characterization of Antiviral Proteins against Bunyaviruses in Mosquito-Derived Cells

Vector Competence Assays for RVFV in Mosquitoes

Experimental Infection of Ticks with SFTSV

Methods for Thrips Transmission and Maintenance of Tomato Spotted Wilt Virus (TSWV)

In Vitro Evaluation of Bunyavirus T-Cell Immunity

Analysis of Leishbuviridae from Trypanosomatids

Recombinant Protein Production and Purification of Rift Valley Fever Virus Nucleoprotein from Escherichia coli Expression Systems

Bunyaviruses: Expression of Recombinant Gycoproteins from Insect Cells

Murine Models to Study Crimean-Congo Hemorrhagic Fever Hepatic Injury

A Review on Reliable and Standardized Animal Models to Study the Pathogenesis of Schmallenberg Virus in Ruminant Natural Host Species

Schmallenberg Virus, From the Necropsy Room to the Laboratory

Reverse Genetics System for Crimean-Congo Hemorrhagic Fever Virus

Recombinant Vaccine Production: Production of a Recombinant CCHF MVA Vaccine

Efficient Expression of Oropouche Virus Nonstructural Proteins NSs and Nsm

Systemic Clodronate Liposome Treatment for Depletion of Blood-Contacting Phagocytes and Investigation of Their Role in the Control of Bunyavirus Viremia.

ISBN:

9781071643389

107164338X