Schizosaccharomyces pombe : Methods and Protocols / edited by Ruben C. Petreaca.

Format:

Book

Contributor:

Petreaca, Ruben C., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2862

Language:

English

Subjects (All):

Microbiology.

Biological models.

Biological Models.

Local Subjects:

Microbiology.

Biological Models.

Physical Description:

1 online resource (XII, 365 p. 65 illus., 38 illus. in color.)

Edition:

1st ed. 2025.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2025.

Summary:

This detailed volume explores techniques utilizing the fission yeast Schizosaccharomyces pombe, a powerful tractable model system for eukaryotic biology. The book begins with a section on microscopy and imaging, and it continues with chapters covering genome and plasmid editing, including the use of CRISPR/Cas9 technologies, as well as the analysis of DNA damage, repair, and recombination, and more. The volume also includes chapters with a focus on computational methods specific to the approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Schizosaccharomyces pombe: Methods and Protocols serves as the ideal guide for researchers in the fission yeast community working with emerging technologies.

Contents:

Dual Luciferase Reporter Assay in Schizosaccharomyces pombe

High-Throughput Measurement of Single Fission Yeast Cell Volume Using Fluorescence Exclusion

Measuring Cell Dimensions in Fission Yeast Using Machine Learning

Ultrastructure Expansion Microscopy (U-ExM) of the Fission Yeast S. pombe

Live Imaging of Fission Yeast Single Cell Lineages Using a Microfluidic Device

Analysis of Cell Wall Mechanics in Fission Yeast

Pil1 Co-Tethering Assay to Detect Protein-Protein Interactions in the Fission Yeast Schizosaccharomyces pombe

Bi-Molecular Fluorescence Complementation as a Tool to Study Specific Dynamic Interactions Between Proteins in Fission Yeast

Mapping Active RNA Polymerases in Proliferating and Quiescent Fission Yeast Cells Using Precision Run-On Sequencing

Fast Fission Yeast Genome Editing by CRISPR/Cas9 Using Gap Repair and Fluoride Selection

CRISPR–Cas9 Genome Editing in Auxotrophic and Non-Auxotrophic Fission Yeast Strains

Proliferation Analyses of Conditional Knockdown Strains Using CRISPR Interference in Fission Yeast

SIMPLE Method for Plasmid Editing

Investigating Fission Yeast Mutagenesis Using Canavanine Sensitivity Assays

A Rapidly Inducible DNA Double-Strand Break to Monitor Telomere Formation, DNA Repair, and Checkpoint Activation

Targeted Forward Genetics: Saturating Mutational Analyses of Specific Target Loci Within the Genome

Assessing Drug Sensitivity in Fission Yeast Using Half-Maximal Inhibitory Concentration (IC50) Assays

ChIPmentation for Epigenomic Analysis in Fission Yeast

Dissecting the Cell-Killing Mechanisms of Hydroxyurea Using Spot Assays

Measuring Meiotic Recombination Frequency in Schizosaccharomyces pombe Using an Engineered Genetic Interval

Label-Free Quantitative Phosphoproteomics in the Fission Yeast Schizosaccharomyces pombe

Genome-Wide Profiling of Histone Modifications in Fission Yeast Using CUT&Tag

Identifying Drug Sensitivities in Fission Yeast by Assessing Growth Kinetics

An “In Schizo” Evaluation System to Screen for Human Kinesin-5 Inhibitors

Automated Machine Learning Tools to Build Regression Models for Schizosaccharomyces pombe Omics Data.

ISBN:

9781071641682