Marburg Virus : Methods and Protocols / edited by Logan Banadyga, Andrea Marzi.

Format:

Book

Contributor:

Banadyga, Logan., Editor.

Marzi, Andrea, Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2877

Language:

English

Subjects (All):

Virology.

Local Subjects:

Virology.

Physical Description:

1 online resource (XVI, 455 p. 54 illus., 51 illus. in color.)

Edition:

1st ed. 2025.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2025.

Summary:

This volume explores the latest advancements in the study of the Marburg virus. The first few chapters in this book review the challenges associated with developing effective medical countermeasures against this virus, while the rest of the chapters provide protocols on ways to achieve these goals. Some of the topics covered in this book include virus isolation and propagation; single-cell transcriptional profiling; reverse genetics systems; proteomics analyses; next-generation sequencing techniques; protocols detailing the use of various animal models including bats and nonhuman primates; and methods for assessing virus stability or validating virus inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Marburg Virus: Methods and Protocols is a valuable resource for all researchers interested in advancing their understanding of this virus.

Contents:

Mismatch of Supply and Demand: Marburg Virus Disease Outbreaks Need Countermeasures but Will Not Provide Opportunity for Clinical Trials

Marburg Virus Medical Countermeasures

Isolation and Propagation of Marburg viruses

Validation of Marburg Virus Inactivation by AVL Buffer

Generation, Recovery, and Propagation of a Recombinant Vesicular Stomatitis Virus Expressing the Marburg Virus Glycoprotein

Visualizing the Internalization of Marburg Virus-Like Particles into Living Cells

Proximity Proteomics to Profile Ebola Virus Protein Interactome in its Functional Context

Monoclonal Antibody Production Against Filoviruses from Immunized Mice

Marburg Virus Minigenome Assays

Transcription- and Replication-Competent Virus-Like Particle Systems for Marburg Virus

Generation of Biologically Contained Marburg Virus

Recovery of Recombinant Marburg Virus by Reverse Genetics

In Vivo Investigation of Filovirus Glycoprotein-Mediated Infection in a BSL2 Setting

Evaluating Countermeasures against Marburg Virus Using a Mouse Model

Filovirus Infection in Humanized Mouse Models

A Hamster Model for the Evaluation of Marburg Virus Countermeasures

Evaluation of Marburg Virus Medical Countermeasures in Guinea Pigs

Experimental Infection of Egyptian Rousette Bats (Rousettus aegyptiacus) with Marburg Virus

Evaluation of Vaccines and Therapeutics Against Ebolaviruses in the Domestic Ferret

Evaluation of Vaccines and Therapeutics against Marburg Virus in Nonhuman Primate Models

Marburg Virus Aerosol Infection in Animal Models

Gross and Histopathologic Evaluation of Tissues from Marburg Virus Infected Nonhuman Primates

Detection of Serum Antibodies Targeting the Marburg Virus Glycoprotein using a Multiplex Immunoassay Platform

Quantification of Neutralizing Antibodies in Serum using VSV-MARV-GFP

Cellular Phenotyping of Peripheral Blood Mononuclear Cells from Marburg Virus-Infected Animals

Transcriptional Profiling of Filovirus-Infected Nonhuman Primate Samples

Droplet-Based Single Cell 3’ mRNA Sequencing of Marburg Virus Infected Samples

Quantification of Filovirus Soluble Glycoprotein using a Sandwich ELISA

Immunochromatographic Lateral Flow Assays to Detect Filovirus Nucleoproteins

Amplification of Filovirus Genomes from Clinical Samples for Next Generation Sequencing

Virus Stability on Surfaces, in Bodily Fluids, in Wastewater, and Different Environmental Conditions.

ISBN:

9781071642566

1071642561