Glaucoma : Methods and Protocols / edited by Tatjana Jakobs, Paloma B. Liton.

Format:

Book

Contributor:

Jakobs, Tatjana., Editor.

Liton, Paloma B., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2858

Language:

English

Subjects (All):

Ophthalmology.

Local Subjects:

Ophthalmology.

Physical Description:

1 online resource (XII, 314 p. 83 illus., 78 illus. in color.)

Edition:

2nd ed. 2025.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2025.

Summary:

This second edition volume expands on the previous edition with an update on the latest methodologies used to study the neurodegeneration of glaucoma. This book notably features a focus on ocular hypertension as an important factor in the pathogenesis of glaucoma, and the relationship between elevated intraocular pressure and neurodegeneration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Glaucoma: Methods and Protocols, Second Edition is a valuable resource for anyone interested in glaucoma research. This book will encourage innovation and facilitate progress toward improving our understanding and treatment of glaucoma.

Contents:

Evaluation of Cross-Linked Actin Networks (CLANs) in Human Trabecular Meshwork Cells and Tissues

3D Extracellular Matrix Protein Hydrogels for Human Trabecular Meshwork Cell Studies

Protocol for the Extraction and Characterization of Trabecular Meshwork Cell Cytoskeletome Fraction

Measurements of Phagocytosis in Trabecular Meshwork Cells

Nanotubules and Cellular Communication in Trabecular Meshwork Cells

Isolation and Characterization of Exosomes from Ocular Samples

Timelapse Live-Cell Imaging Using Fluorescent Protein Sensors in Outflow Pathway Cells Under Fluid Flow Conditions

Human Anterior Segment Perfusion Organ Culture

Segmental Aqueous Humor Outflow

Human Corneal Rim Perfusion Culture

The Inducible TGFβ2 Ocular Hypertension Mouse Model

Mouse Model of Glucocorticoid-Induced Glaucoma

Trabecular Meshwork Stem Cells for Glaucoma Treatment

Aqueous Humor Angiography

Retinal Ganglion Cell Transplantation: Differentiation and Quantification Strategies

Retinal Ganglion Cell Subtypes and Their Vulnerability in Glaucoma

Full-Field Electroretinogram Responses in Rodent Models of Ganglion Cell Injury

Testing Visual Function by Assessment of the Optomotor Reflex in Glaucoma

Controlled Elevation of Intraocular Pressure (CEI) Glaucoma Model in Rats

A Comprehensive Protocol for Microbead-Induced Ocular Hypertension in Mice

Continuous Wireless Telemetric Measurement of Intraocular Pressure (IOP), Ocular Perfusion Pressure (OPP), and Cerebrospinal Fluid Pressure (CFP) in Nonhuman Primates (NHPs)

In Vitro and In Vivo Methods for Studying Retinal Ganglion Cell Survival and Optic Nerve Regeneration.

ISBN:

9781071641408

1071641409