Clinical Metabolomics : Methods and Protocols / edited by Martin Giera, Elena Sánchez-López.

Format:

Book

Contributor:

Giera, Martin, Editor.

Sánchez-López, Elena., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2855

Language:

English

Subjects (All):

Biochemistry.

Metabolism.

Cytology.

Pharmacology.

Metabolic Pathways.

Local Subjects:

Metabolic Pathways.

Pharmacology.

Physical Description:

1 online resource (XV, 575 p. 119 illus., 86 illus. in color.)

Edition:

2nd ed. 2025.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2025.

Summary:

This second edition volume presents new and updated protocols on comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations. Chapters explore the core of several promising initiatives evolving around personalized health care and precision medicine. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Clinical Metabolomics: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .

Contents:

(Pre)Clinical Metabolomics Analysis

Global Metabolomics using LC-MS for Clinical Applications

Modular, scalable and customizable LC-HRMS for exposomics

Distinguishing artifactual fatty acid dimers from FAHFAs in untargeted LC-MS pipelines

Advances in Chiral Metabolomic Profiling and Biomarker Discovery

Stable Isotope Tracing Experiments using LC-MS

Quantification of Total Ketone Bodies in biological matrices using Stable Isotope-labeled Internal Standards and RP-UPLC-MS/MS

LC-MS/MS quantification of endocannabinoids in tissues

Prostaglandin Metabolites Analysis in Urine by LC-MS/MS

A method for analysis of oxidized phospholipids from biological samples using mass spectrometry

Non-enzymatic oxidized polyunsaturated fatty acid products in human plasma and urine samples by LC-QTOF-MS/MS

Reversed-Phase Ultrahigh-Performance Liquid Chromatography – Mass Spectrometry Method for High-Throughput Lipidomic Quantitation

High-Throughput RPLC-MS/MS Quantification of Short- and Medium-Chain Fatty Acids

Long chain base profiling with multiple reaction monitoring mass spectrometry

Sphingolipid analysis in clinical research

Recommendations for accurate lipid annotation and semi-absolute quantification from LC-MS/MS datasets

Rapid analysis of Sterols in Blood Derived Samples

Ultrahigh-Performance Supercritical Fluid Chromatography – Mass Spectrometry in the Clinical Lipidomic Analysis

Quantification of octadecanoids in human plasma using chiral supercritical fluid chromatography-tandem mass spectrometry

Rapid Lipid Mediator Profiling by Convergence Chromatography-MS/MS

Analysis of the Mammalian Lipidome by DMS Shotgun Lipidomics

Differential mobility spectrometry-based cardiolipin analysis

CE-MS-based clinical metabolomics of human plasma

Quantitative blood serum IVDr NMR spectroscopy in clinical metabolomics of cancer, neurodegeneration and internal medicine

Lipoprotein (sub)fraction analysis on the Bruker B.I. LISA platform

NMR-based stable isotope tracing of cancer metabolism

NMR-based analysis of cellular central energy metabolism and exchange ratesIn Situ Isotope

Tracing at Single-Cell Resolution Using Mass Spectrometry Imaging

Extracting knowledge from MS clinical metabolomic data: Processing and analysis strategies

Application of a computational metabolomics workflow for the diagnosis of inborn errors of metabolism in a laboratory setting.

ISBN:

9781071641163

1071641166