G4 and i-motif biology / edited by Kevin D. Raney, Robert L. Eoff, Alicia K. Byrd, and Samantha Kendrick.

Format:

Book

Contributor:

Raney, Kevin D. (Kevin Douglas), editor.

Eoff, Robert L., editor.

Byrd, Alicia K., editor.

Kendrick, Samantha, editor.

Series:

Methods in enzymology ; 695.

Methods in enzymology, 0076-6879 ; 695

Language:

English

Subjects (All):

G proteins.

DNA.

RNA.

Physical Description:

xxi, 274 pages : color illustrations ; 24 cm.

Place of Publication:

Cambridge, MA : Academic Press, 2024.

Summary:

"The chapters assembled in this Methods in Enzymology volume are an attempt to highlight some of the most exciting methods available to researchers studying the chemistry and biology of non-canonical nucleic acids, especially four-stranded G-quadruplex (G4) and i-motif (iM) structures."--Preface.

Contents:

Purification and biochemical characterization of the G4 resolvase and DNA helicase FANCJ / Tomasz Kulikowicz, Joshua A. Sommers, Kathleen F. Fuchs, Yuliang Wu, and Robert M. Brosh Jr.

In-gel straining methods of G4 DNA and RNA structures / Philipp Schult and Katrin Paeschke

A beginner's handbook to identify and characterize i-motif DNA / Pallabi Sengupta, Jan Jamroskovic, and Nasim Sabouri

Single-molecule observation of G-quadruplex and R-loop formation induced by transcription / Jihee Hwang, Bradleigh Palmer, and Sua Myong

Advancing the study of protein-G4 interactions in DNA repair: insights from biolayer interferometry / Kaitlin Lowran, Vereena Salib, Emma Cismas, and Colin G. Wu

Immunofluorescence microscopy of G-quadruplexes and R-loops / Giulia Miglietta, ,Jessica Marinello, and Giovanni Capranico

Observing G4 formation and its resolution by Pif1 in real time by manipulation under magnetic tweezers / Jessica valle-Orer̦o, Martin Rieu, Jean-Franc̦ois Allemand, Dulamkhuu Bujaa, ALaexandra Joubert, Phong Lan Thao Tran, Vincent Croquette, and Jean-Baptiste Boulé

In vivo detection of DNA secondary structures using permanganate/S1 footprinting with direct adapter ligation and sequencing (PDAL-Seq) / Angelika Lahnsteiner, Sarah J.C. Craig, Kaivan Kamali, Bernadette Weissensteiner, Barbara McGrath, Angela Risch, and Kateryna D. Makova

Production of the anti-G-quadruplex antibody BG4 for efficient genome-wide analyses: from plasmid quality control to antibody validation / Ilaria Maurizio, Beatrice Tosonii, Irene Gallina, Emanuela Ruggiero, Irene Zanin, and Sara N. Richter

Utility of intercalator displacement assays for screening of ligands for i-motif DNA structures / Dilek Guneri and Zoë A.E. Waller

Potentiometric titrations to study ligand interactions with DNA i-motifs / Joseph Boissieras and Anton Granzhan

Identification of bona fide RNA G-quadruplex binding proteins / Prakash Kharel and Pavel Ivanov.

Notes:

Includes bibliographical references.

ISBN:

0443217742

9780443217746

OCLC:

1396691569