Human Induced Pluripotent Stem Cells / edited by Baojin Ding, Yu Tang.

Format:

Book

Contributor:

Ding, Baojin, Editor.

Tang, Yu, Editor.

Series:

Neuromethods, 1940-6045 ; 210

Language:

English

Subjects (All):

Neurosciences.

Stem cells.

Neuroscience.

Stem Cell Biology.

Local Subjects:

Neuroscience.

Stem Cell Biology.

Physical Description:

1 online resource (XIII, 252 p. 57 illus., 50 illus. in color.)

Edition:

1st ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This volume explores the latest developments in the field and showcases the vast potential of human induced pluripotent stem cells (hiPSCs) in advancing neuroscience research. The chapters in this book are organized into three parts. Part One describes the generation, characterization, and validation of hiPSCs using different methodologies from various resources. Part Two focuses on techniques for genome editing of hiPSCs. Part Three covers various hiPSC applications in neuroscience research and strategies for analyzing hiPSC-based model systems. These applications include the generation of neural subtypes, glial cells, and skeletal muscles; assembling co-culture systems to prepare highly pure neurons at late maturation stages; measuring nuclear transport of proteins and transcripts in cultured neurons; and generating brain organoids. In the Neuromethods series style, chapters include the kind of detail and key advice from specialists needed to achieve successful results in your laboratory. Cutting-edge and comprehensive, Human Induced Pluripotent Stem Cells is a valuable resource for researchers and students in neuroscience, neurology, stem cell biology, and other related fields.

Contents:

Generation and Characterization of Human Induced Pluripotent Stem Cells via Retroviral Delivery of Yamanaka Factors

Generation of Human Induced Pluripotent Stem Cells via Episomal Vectors

Generation and Characterization of Human Induced Pluripotent Stem Cells from Urine-Derived Cells

Gene Editing and Reprogramming of Human Fibroblast Cells to Human Induced Pluripotent Stem Cells

Validation of Pluripotency of Human Induced Pluripotent Stem Cells

Generation of Knockout Human PSCs using CRISPR/Cas9 Editing with a Donor Template

Generation of Reporter Human Pluripotent Stem Cells using CRISPR/Cas9 Editing

Deletion of Large Intergenic Chromosomal Fragments in Human Induced Pluripotent Stem Cells (hiPSC) by CRISPR/Cas9 Genome Editing

Gene Editing in Human Induced Pluripotent Stem Cell Using Prime Editing

Lentiviral Delivery of Transcription Factors to Generate Motor Neurons from Human Induced Pluripotent Stem Cells

Generation of Motor Neurons from Human Induced Pluripotent Stem Cells using Small Chemical Molecules

Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) into Mature and Contractible Skeletal Muscles

Generation of Functional Microglia from Human Pluripotent Stem Cells

Assembling an Indirect Coculture System for Preparation of Highly Pure hiPSC-Derived Motor Neurons at Late-Stage Maturation Stages

Measuring Protein Nuclear Transport via a Dual Reporter in iPSC-Derived Neurons

Nuclear mRNA Export Measurement using FISH in iPSC-Derived Neurons

Integrated Approach for the Differentiation of Neurons and Astrocytes from Human Pluripotent Stem Cells

Generation of Human iPSC-Derived Cerebellar Organoids

Cortical-Organoid Grafted Microvascular Bed on a Chip.

ISBN:

9781071639993

1071639994