Antibiotic Resistance Protocols / edited by Stephen H. Gillespie.

Format:

Book

Contributor:

Gillespie, S. H., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2833

Language:

English

Subjects (All):

Biology--Technique.

Biology.

Experimental immunology.

Microbiology.

Bacteria.

Immunological Techniques.

Local Subjects:

Immunological Techniques.

Microbiology.

Bacteria.

Physical Description:

1 online resource (XI, 227 p. 31 illus., 13 illus. in color.)

Edition:

4th ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This fully updated edition explores current techniques for research into antibiotic resistance. The book begins with how samples are collected, strains isolated and sequenced, and the results integrated in the microbiological workflow. It continues with novel methods to test resistance and interactions between antibiotics, physiological conditions, or using innovative tools like the hollow fiber or Raman spectroscopy, as well as mathematical models that can describe resistance within host. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Antibiotic Resistance Protocols, Fourth Edition serves as an ideal guide for answering questions on how to control antibiotic resistance, to develop new agents, and to address the problems posed by microbes that have become resistant to our antibiotics.

Contents:

Using the Zebrafish Larval Model of Infection to Investigate Antibiotic Efficacy and Combination Treatments Against Staphylococcus aureus

Three-Dimensional Rotary Culture to Model Mycobacterial Biofilms in Low-Shear Detergent-Free Liquid Suspension

Rapid Gene Silencing Followed by Antimicrobial Susceptibility Testing for Target Validation in Antibiotic Discovery

Modified HT-SPOTi: An Antimicrobial Susceptibility Testing to Evaluate Formulated Therapeutic Combinations Against Bacterial Growth and Viability

Investigating Combination Therapy as a Means to Enhance Activity and Repurpose Antimicrobials

Investigating Photoactive Antimicrobials as Alternatives (or Adjuncts) to Traditional Therapy

Using Hollow Fiber to Model Treatment of Antimicrobial Resistant Organisms

A Microtitre Plate Assay at Acidic pH to Identify Potentiators that Enhance Pyrazinamide Activity Against Mycobacterium tuberculosis

Within-Host Mathematical Models of Antibiotic Resistance

Use of Individual-Based Mathematical Modelling to Understand More About Antibiotic Resistance Within-Host

Monitoring Live Mycobacteria in Real-Time Using a Microfluidic Acoustic-Raman Platform

Phylogenetic Survival Analysis

Rapid Drug Susceptibility Testing to Preserve Antibiotics

Quantifying Viable M. tuberculosis Safely Obviating the Need for High Containment Facilities

Improved Diagnosis and Treatment Monitoring Tuberculosis Using Stool and the Tuberculosis Bacterial Load Assay (TB-MBLA)

Application of Pathogen Genomics to Outbreak Investigation

Use of Whole Genome Sequencing for Mycobacterium tuberculosis Complex Antimicrobial Susceptibility Testing

Use of Whole Genome Sequencing for Mycobacterium tuberculosis Complex Antimicrobial Susceptibility Testing: From Sequence Data to Resistance Profiles

Analysis of Whole Genome Sequencing Data for Detection of Antimicrobial Resistance Determinants.

ISBN:

9781071639818