Plant Cell Culture Protocols / edited by Víctor Loyola-Vargas, Neftalí Ochoa-Alejo.

Format:

Book

Contributor:

Loyola-Vargas, Víctor., Editor.

Ochoa-Alejo, Neftalí, Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2827

Language:

English

Subjects (All):

Botany.

Plant Science.

Local Subjects:

Plant Science.

Physical Description:

1 online resource (XV, 457 p. 118 illus., 105 illus. in color.)

Edition:

5th ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This fifth edition provides new and updated protocols on plant cell, tissue, and organ cultures. Chapters are divided into five parts that cover topics from general methodologies, statistical analysis and contamination control, highly specialized techniques, and laborious process of measuring the epigenetics changes in tissue cultures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Cell Culture, Fifth Edition aims to ensure successful results in the further study of this vital field.

Contents:

An introduction to plant cell, tissue, and organ culture: current status and perspectives

Use of Statistics in Plant Cell Tissue Culture

Plant Micropropagation and Temporary Immersion Systems

The History of Agrobacterium rhizogenes. From Pathogen to a Multitasking Platform for Biotechnology

Use of Electron Microscopy for the Detection of Contaminant Endophytic Bacteria in vitro

Using Mathematical Optimization Models to Improve the Efficiency of Duckweeds (Wolffia arrhiza and Lemna minor) Micropropagation

Application of Artificial Neural Network and Genetic Algorithm Modeling for in vitro Regeneration of Seaweed Seedling Production

Less Frequently Used Growth Regulators in Plant Tissue Culture

Protocol for Generating Arabidopsis thaliana Cell Suspension Cultures Under Different Light Conditions

Micropropagation of Agave angustifolia “Espadín” Used in the Production of Mezcal

In Vitro Propagation of Threatened Agave Species Using a Two-Stage System

In vitro Propagation of "Salsaparrilha" [Herreria salsaparilha Mart. (Asparagaceae)]: an Anti-syphilitic Medicinal Species

Micropropagation of the Ornamental Aquatic Plant, Aponogeton ulvaceus, from Immature Tuber Explants

Protocol for the Acclimatization of Coconut Vitro-plants Under ex-vitro Conditions

Advances in tissue culture and transformation studies in non-model species: Passiflora spp. (Passifloraceae)

Advances in tissue culture and transformation studies in non-model species: Bixa orellana L. (Bixaceae)

Another Culture Protocols for Barley and Wheat

Synthetic tetraploid of Oncidium crispum Lodd. (Orchidaceae)

Direct Somatic Embryogenesis in Carica papaya L. genotypes for Genetic Modification Purposes

Scale-up of Coffea canephora Somatic Embryogenesis In Temporary Immersion System

Large-scale Production of Specialized Metabolites in vitro Cultures

Quantification of DNA methylation by ELISA in epigenetic studies in plant tissue culture: a theoretical-practical guide

Proteomic Analysis Of in vitro Plant Tissues: Coffea canephora A Case Of Study

Transcriptomic Analysis During the Induction of Somatic Embryogenesis in Coffea canephora

Chloroplast isolation in agavaceas

Overexpression of Genes to Enhance Abiotic Stress Tolerance in Arabidopsis

Plant Tissue Culture and Metabolite Profiling for High-Value Natural Product Synthesis

A Novel Method For in situ RT-PCR Based on Capsules From Centrifuge Tubes, Ideal for Transcripts Detection in Plant Tissues

Isolation of Protoplasts from Tissues of Mexican Prickly Poppy (Argemone mexicana L.). An Alkaloid-Producing Medicinal Plant.

ISBN:

9781071639542