Photorespiration : Methods and Protocols / edited by Berkley J Walker.

Format:

Book

Contributor:

Walker, Berkley J., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2792

Language:

English

Subjects (All):

Botany.

Plant Science.

Local Subjects:

Plant Science.

Physical Description:

1 online resource (XI, 278 p. 57 illus., 41 illus. in color.)

Edition:

1st ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This volume provides methods on researching photorespiration to provide a better understanding of this vital pathway in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Photorespiration: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

High throughput phosphoglycolate phosphatase activity assay using crude leaf extract and recombinant enzyme to determine kinetic parameters Km and Vmax using a microplate reader- Measuring phosphoglycolate phosphatase activity using NMR detection of glycolate

Determination of phosphoglycolate phosphatase activity via a coupled reaction using recombinant glycolate oxidase

Spectrophotometric Assays for Measuring Photorespiratory Glutamate:Glyoxylate and Serine:Glyoxylate Aminotransferase Reactions

Mitochondrial dihydrolipoamide dehydrogenase (mtLPD1) – expression, purification, activity and redox regulation.-Spectrophotometric Assays for Measuring Hydroxypyruvate Reductase Activity

High throughput glycerate kinase activity assay using crude leaf extract and recombinant enzyme to determine kinetic parameters Km and Vmax using a microplate reader

Purification of recombinant N-terminal histidine-tagged Arabidopsis thalianaphosphoglycolate phosphatase 1, glycolate oxidase 1 and 2, and hydroxypyruvate reductase 1

Measuring and Quantifying Characteristics of the Post-Illumination Burst

Low-cost system for maintaining low atmospheric CO2 levels during Arabidopsis cultivation

Measuring the release of ammonia from leaves

Using gas exchange to study CO2 release during photosynthesis with steady- and non-steady-state approaches

Measurement of photorespiratory oxygen exchange by membrane inlet mass spectrometry

Using Dynamic Gas Exchange Measurements During Oxygen Transients to Study Non-steady-state Photorespiration

Analysis of photorespiratory intermediates under transient conditions by mass spectrometry

Combining gas exchange and rapid quenching of leaf tissue for mass spectrometry and NMR analysis using an external chamber

Combining gas exchange and rapid quenching of leaf tissue for mass spectrometry analysis directly in gas exchange cuvette

Reaction-kinetic modeling of photorespiration using modelbase

Using publicly available RNA-seq data for expression analysis of genes of interest

Identification and functional characterization of cis-regulatory elements of key photorespiratory genes in response to short-term abiotic stress conditions

Identification of auxiliary organellar targeting signals for plant peroxisomes using bioinformatic analysis of large protein sequence datasets followed by experimental validation.

ISBN:

9781071638026

1071638025