Coupled enzyme activity and thermal shift screening of the Maybridge Rule of 3 Fragment Library against Trypanosoma brucei choline kinase, a genetically validated drug target / Louise L. Major, Helen Denton, Terry K. Smith.

Format:

Journal/Periodical

Author/Creator:

Major, Louise L., author.

Denton, Helen, author.

Smith, Terry K., author.

Language:

English

Subjects (All):

Fluorimetry.

Trypanosoma.

Physical Description:

1 online resource

annual

Began with: 2013-

Place of Publication:

Rijeka (HR) : InTech, 2013.

Summary:

In this study the authors interrogate ~630 compounds of the Maybridge Rule of 3 Fragment Library for compounds that interact with, and inhibit TbCK. The Maybridge Rule of 3 Fragment Library is a small collection of quantifiable diverse [35, 36], pharmacophoric rich, chemical entities. Comparisons between two different screening methods, a coupled enzyme activity assay and differential scanning fluorimetry, has allowed identification of compounds that interact and inhibit the T. brucei choline kinase, several of which possess selective trypanocidal activity.

Contents:

14. Coupled Enzyme Activity and Thermal Shift Screening of the Maybridge Rule of 3 Fragment Library Against Trypanosoma brucei Choline Kinase; A Genetically Validated Drug Target

Louise L. Major, Helen Denton, and Terry K. Smith.

1. Introduction

2. Experimental

3. Results and discussion

4. Conclusions

Acknowledgements

References.

Notes:

Description based on publisher supplied metadata and other sources.