Helicase enzymes / edited by Michael A. Trakselis.

Format:

Book

Contributor:

Trakselis, Michael A., editor.

Series:

Methods in enzymology ; 0076-6879 v. 672-673.

Methods in enzymology, 0076-6879 ; volume 672-673

Language:

English

Subjects (All):

Enzymes.

Enzymology.

DNA helicases.

Enzymes--genetics.

DNA Helicases.

RNA Helicases.

enzyme.

Medical Subjects:

Enzymes--genetics.

DNA Helicases.

RNA Helicases.

Enzymes.

Physical Description:

2 volumes : illustrations ; 23 cm.

Edition:

First edition.

Place of Publication:

Cambridge, MA : Elsevier/Academic Press, 2022.

Contents:

Part A. Monitoring helicase-catalyzed unwinding of multiple duplexes simultaneously / Matthew D. Thompson, Emory G. Malone, and Alicia K. Byrd ; Alignment of helicases on single-stranded DNA increases activity / Deniz Ozaslan, Alicia K. Byrd, Binyam Belachew, and Kevin D. Raney ; Use of an unnatural amino acid to map helicase/DNA interfaces via photoactivated crosslinking / Alexander T. Duckworth and James L. Keck ; Quantitative methods to study helicase, DNA polymerase, and exonuclease coupling during DNA replication / Anupam Singh and Smita S. Patel ; Assembling bacteriophage T7 leading-strand replisome for structural investigation / Chen-Yu Lo and Yang Gao ; In vivo fluorescent TUNEL detection of single stranded DNA gaps and breaks induced by dnaB helicase mutants in Escherichia coli / Megan S. Behrmann and Michael A. Trakselis ; Biochemical methods to monitor loading and activation of hexameric helicases / Amy J. Fernandez and James M. Berger ; DNA fiber analyses to study functional importance of helicases and associated factors during replication stress / Arindam Datta and Robert M. Brosh ; Optimizing CMG helicase and CMG-dependent replication assays by designing DNA fork substrates and choosing nucleotide analogues for helicase preloading / Nina Y. Yao and Mike E. O'Donnell ; ReconSil : An electron microscopy toolbox to study helicase function at an origin of replication / Thomas Pühringer, Julia F. Greiwe, Thomas C.R. Miller, and Alessandro Costa ; CMG helicase activity on G4-containing DNA templates / Sahil Batra, Sujan Devbhandari, and Dirk Remus ; Detecting G4 unwinding / Stefan Juranek and Katrin Paeschke ; Helicase mediated vectorial folding of telomere G-quadruplex / Tapas Paul and Sua Myong ; Production of long linear DNA substrates with site-specific chemical lesions for single-molecule replisome studies / Gurleen Kaur, Lisanne M. Spenkelink, Jacob S. Lewis, Slobodan Jergic, Nicholas E. Dixon, and Antoine M. van Oijen ; Replication fork collapse in vitro using Xenopus egg extracts / Sara C. Conwell, Matthew T. Cranford, Tamar Kavlashvili, and James M. Dewar ; Measuring the contributions of helicases to break-induced replication / Zhenxin Yan, Liping Liu, Nhung Pham, Pilendra K. Thakre, Anna Malkova, and Grzegorz Ira ; A RADAR method to measure DNA topoisomerase covalent complexes / Alice Meroni and Alessandro Vindigni

Part B. Fluorescence approaches for biochemical analysis of ATP-dependent chromatin remodeling enzymes / Alexander S. Baier and Craig L. Peterson

Attacking a DEAD problem : The role of DEAD-box ATPases in ribosome assembly and beyond / Katrin Karbstein

Monitoring functional RNA binding of RNA-dependent ATPase enzymes such as SF2 helicases using RNA dependent ATPase assays : A RIG-I case study / Rong Guo and Anna Marie Pyle

Measurement of ATP utilization in RNA unwinding and RNA chaperone activities by DEAD-box helicase proteins / Inga Jarmoskaite, Anna E. Helmers, and Rick Russell

In vitro characterization of Dhr1 from Saccharomyces cerevisiae / Ran Lin, Carl C. Correll, and Arlen W. Johnson

Translational control by helicases during cellular stress / Sara B. Carey and Timothy A. Bolger

Lysate and cell-based assays to probe the translational role of RNA helicases / Kevin C. Wilkins, Srivats Venkataramanan, and Stephen N. Floor

Bulk phase biochemistry of PIF1 and RecQ4 family helicases / Prasangi Rajapaksha, Robert H. Simmons, Spencer J. Gray, David J. Sun, Phoebe Nguyen, David G. Nickens, and Matthew L. Bochman

Biochemical and single-molecule techniques to study accessory helicase resolution of R-loop proteins at stalled replication forks / Alisa E. Shaw, Sreeya Kairamkonda, Harshad Ghodke, and Grant D. Schauer

Transcriptional regulation by a RecQ helicase / Subrata Debnath, Xing Lu, and Sudha Sharma

Analysis of the conformational space and dynamics of RNA helicases by single-molecule FRET in solution and on surfaces / Dagmar Klostermeier

Long DNA constructs to study helicases and nucleic acid translocases using optical tweezers / Clara Aicart-Ramos, Silvia Hormeno, Oliver J. Wilkinson, Mark S. Dillingham, and Fernando Moreno-Herrero

Novel approaches to study helicases using magnetic tweezers / Nadia Ruiz-Gutierrez, Martin Rieu, Jimmy Ouellet, Jean-François Allemand, Vincent Croquette and Hervé Le Hir

Reconstitution and biochemical characterization of the RNA-guided helicase-nuclease protein Cas3 from type I-A CRISPR-Cas system / Chunyi Hu and Ailong Ke

Purification and characterization of Mtr4 and TRAMP from S. cerevisiae / Matthew K. Yim, Joshua M. Denson, Mark D. Gold, and Sean J. Johnson

Methods to assess helicase and translocation activities of human nuclear RNA exosome and RNA adaptor complexes / M. Rhyan Puno and Christopher D. Lima

Using hydrogen-deuterium exchange mass spectrometry to characterize Mtr4 interactions with RNA / Darby Ball, Trang Nguyen, Naifu Zhang, and Sheena D'Arcy.

Notes:

Includes bibliographical references.

Other Format:

ebook version :

ISBN:

9780323914765

0323914764

9780323997737

0323997732

OCLC:

1332952896