Thermomorphogenesis : Methods and Protocols / edited by Meng Chen.

Format:

Book

Contributor:

Chen, Meng., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2795

Language:

English

Subjects (All):

Botany.

Stress (Physiology).

Plants.

Plant Science.

Plant Stress Responses.

Local Subjects:

Plant Science.

Plant Stress Responses.

Physical Description:

1 online resource (XI, 264 p. 60 illus., 53 illus. in color.)

Edition:

1st ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This detailed collection explores thermomorphogenesis, a discipline that unravels the intricacies of warm temperature responses in plants. How plants perceive, interpret, and respond to elevated temperatures has far-reaching implications for agriculture, ecology, and our fundamental understanding of plant development, and this book presents techniques to help researchers delve into this area of study. Beginning with a section on thermomorphogenesis responses, the volume continues with numerous chapters on temperature sensing and temperature signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Thermomorphogenesis: Methods and Protocols serves as an ideal guide to this vital subject for research laboratories, as well as for graduate and undergraduate laboratory courses in plant molecular biology.

Contents:

A Guide to Quantify Arabidopsis Seedling Thermomorphogenesis at Single Timepoints and by Interval Monitoring

Dissection of Daytime and Nighttime Thermoresponsive Hypocotyl Elongation in Arabidopsis

Light-Dependent High Ambient Temperature-Induced Senescence Assay Using Whole Seedlings

Assessing Temperature Responses in Roots

A Luciferase Imaging-Based Assay for Studying Temperature Compensation of the Circadian Clock

Investigating the Effects of Temperature on Pathogen Propagation in Arabidopsis

Assessing the High Temperature Effects on Stomatal Production

Characterizing the Synergistic Response Induced by Warm Temperatures and Shade

Measurement of Phytochrome B Thermal Reversion Rates In Vivo

Characterization of Thermoresponsive Photobody Dynamics

Phase Separation of Phytochrome B in HEK293T Cells

Isolation of Phytochrome B Photobodies

Analysis of Phase Separation of EARLY FLOWERING 3

In Vitro Determination of Temperature-Dependent DNA-Binding of the Evening Complex Using Electrophoretic Mobility Shift Assays

Monitoring Real-Time Temperature Dynamics of a Short RNA Hairpin Using Förster Resonance Energy Transfer and Circular Dichroism

Characterization of PIF4 Phosphorylation by SPA1

Chromatin Immunoprecipitation to Investigate H2A.Z Dynamics in Response to Environmental Changes

Assessing the Function of CBF1 in Modulating the Interaction between Phytochrome B and PIF4

GST Pull-Down Assay to Study PIF4 Binding In Vitro

Investigating Circadian Gating of Temperature Responsive Genes

RuBisCO Depletion and Subcellular Fractionation for Enhanced Florigen Detection in Arabidopsis

Identifying Causal Genes for Thermo-Responsive Rosette Growth in Arabidopsis by Genome-Wide Association Study

Mathematical Modeling of Photo- and Thermomorphogenesis in Plants.

ISBN:

9781071638149

1071638149