Plant Functional Genomics : Methods and Protocols, Volume 2 / edited by Fatemeh Maghuly.

Format:

Book

Contributor:

Maghuly, Fatemeh., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2788

Language:

English

Subjects (All):

Plant genetics.

Plant Genetics.

Local Subjects:

Plant Genetics.

Physical Description:

1 online resource (XIV, 413 p. 1 illus.)

Edition:

1st ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This detailed book gathers hands-on techniques to characterize, identify, localize, profile, and determine genome functions in plants. Volume 2 opens with metabolomic methods that uncover chemical intricacies, exploring glycome mixtures, cell wall components, and computational metabolomics, and it continues with sections on plant genome validation, computational approaches and bioinformatic tools, and case studies exploring the biological effects of magnetic fields and the study of cyanobacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Plant Functional Genomics: Methods and Protocols serves as an ideal guide to the immense promise of functional genomics, spanning from fundamental evolutionary studies to practical advancements in crop improvement. Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Contents:

Carotenoid Extraction from Plant Tissues

Sample Preparation from Plant Tissue for Gas Chromatography–Mass Spectrometry (GC–MS)

Identification of Volatiles in Tomato Fruit Using Headspace Solid-Phase-Micro-Extraction (HS-SPME) Coupled with Gas Chromatography-Mass Spectrometry (GC-MS)

Determination of Molecular Dimensions of Carbohydrate Polymers (Polysaccharides) by Size Exclusion Chromatography (SEC)

Monosaccharide Analysis after One-Pot Derivatization Followed by Reverse-Phase Liquid Chromatography Separation and UV/Vis Detection

Atomic Force Microscopy in the Characterization of the Structure of Cell Wall Components

Studying Plant Specialized Metabolites Using Computational Metabolomics Strategies

pyPGCF: A Python Software for Phylogenomic Analysis, Species Demarcation, Identification of Core and Fingerprint Proteins of Bacterial Genomes that Are Important for Plants

Application of Integrated Computational Approaches in Prediction of Plant Virus Encoded miRNAs and Their Targeted Plant Genes

Gene Co-Expression Network Analysis to Mine Genes Involved in Plant Secondary Metabolite Biosynthesis and Regulation

Organic Supplementation of Vaccinium corymbosum Micropropagation Media

Coffee Cell Suspensions as a Platform for Transient Gene Expression Analysis

Agroinfiltration for Enhanced Transgene Expression in Coffee Leaves (Coffea arabica L.)

In Vitro Gamma Mutagenesis Techniques in Rice (Oryza sativa L. var. Lazarroz FL)

CRISPR/Cas9-Mediated Genome Editing in Indica Rice (Oryza sativa L. subsp. indica var. CR-5272)

Dual-Luciferase Reporter Assay for Pre-Screening CRISPR (d)Cas9-Mediated Epigenetic Editing on a Plant Promoter Using Human Cells

In Silico Design of gRNA for CRISPR/Cas9-Mediated Gene Knock-Out

CRISPR/Cas9 Vector Construction for Gene Knock-Out

A Comprehensive Protocol for Assembly of Multiple gRNAs into a Direct Vector for Genome Editing in Tomato

Genome Editing in Brassica juncea Using CRISPR/Cas9 Technology

Precision Genome Editing with CRISPR-Cas9

Using Magnetic Fields to Enhance the Seed Germination, Growth, and Yield of Plants

Phenotypic and Genotypic Methods for the Identification and Quantification of Cyanobacteria in Lake Water.

ISBN:

9781071637821

1071637827