Wolbachia : Methods and Protocols / edited by Ann M. Fallon.

Format:

Book

Contributor:

Fallon, Ann M., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2739

Language:

English

Subjects (All):

Immunology.

Bacteria.

Local Subjects:

Immunology.

Bacteria.

Physical Description:

1 online resource (X, 380 p. 73 illus., 58 illus. in color.)

Edition:

1st ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This book provides insight into the biology of Wolbachia with a particular focus on methodology. Chapters guide readers through reproductive phenotypes caused by Wolbachia, survival and replication of Wolbachia in insect cell lines, artificial introduction of Wolbachia into novel hosts, and analysis of Wolbachia gene functions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Contents:

Wolbachia: Advancing into a second century

Investigation of cytoplasmic incompatibility patterns in Culex pipiens: from field samples to laboratory isofemale lines

Maintenance and Evaluation of Wolbachia Male-Killers of Dipterans

Examining Wolbachia-Induced Parthenogenesis in Hymenoptera

Pillbug: a toolbox for feminisation

Detection, Quantification and Elimination of Wolbachia in Bed Bugs

Detection and analysis of Wolbachia in plant-parasitic nematodes and insights into Wolbachia evolution

Developing Wolbachia-infected cell lines from insects

Use of antibiotics to eliminate Wolbachia from mosquitos and cell culture

Preparation of infectious Wolbachia from a mosquito cell line

Wolbachia transinfection via embryonic microinjection

Measuring host fitness effects and transmission of Wolbachia strains in Aedes aegypti mosquitoes

Detection of natural Wolbachia strains in Anopheles mosquitoes

Procedures for detection of Wolbachia-conferred antiviral protection in Drosophila melanogaster

Validating a mitochondrial sweep accompanying the rapid spread of a maternally-inherited symbiont

Screening host genomic data for Wolbachia infections

A worked example of screening genomic material for the presence of Wolbachia infection

Use of Drosophila Transgenics to Identify Functions for Symbiont Effectors

Using Baker’s Yeast to Determine Functions of Novel Wolbachia (and other prokaryotic) Effectors

Isolation of Phage WO Particles from Wolbachia-infected Arthropods

A light in the dark: Uncovering Wolbachia-host interactions using fluorescence imaging.

ISBN:

9781071635537

1071635530