Drosophila Oogenesis : Methods and Protocols / edited by Michelle S. Giedt, Tina L. Tootle.

Format:

Book

Contributor:

Giedt, Michelle S., Editor.

Tootle, Tina L., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2626

Language:

English

Subjects (All):

Biological models.

Developmental biology.

Biological Models.

Developmental Biology and Stem Cells.

Local Subjects:

Biological Models.

Developmental Biology and Stem Cells.

Physical Description:

1 online resource (XIII, 447 p. 92 illus., 82 illus. in color.)

Edition:

1st ed. 2023.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

Summary:

This detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing techniques from assessing cytoskeletal structures to uncovering protein-protein interactions, with closing chapters exploring how Drosophila oogenesis can be used in the classroom and in outreach programs to increase interest in biomedical research, STEM education, and STEM careers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Drosophila Oogenesis: Methods and Protocols serves as an ideal guide for both new and expert researchers working with this robust and versatile model organism.

Contents:

The Vast Utility of Drosophila Oogenesis

Visualization and Quantification of Drosophila Larval Ovaries

Dissection, Fixation, and Standard Staining of Adult Drosophila Ovaries

Utilizing the FLP-Out System for Clonal RNAi Analysis in the Adult Drosophila Ovary

Analysis of Physiological Control of Adult Drosophila Oogenesis by Inter-Organ Communication

Immunohistochemical Analysis of Nuclear Lamina Structures in the Drosophila Ovary Using CRISPR-Tagged Genes

Visualizing Fusome Morphology via Tubulin Immunofluorescence in Drosophila Ovarian Germ Cells

Tracking Follicle Cell Development

Optimized Fixation and Phalloidin Staining of Basally Localized F-Actin Networks in Collectively Migrating Follicle Cells

Quantitative Image Analysis of Dynamic Cell Behaviors during Border Cell Migration

Live Imaging of Nurse Cell Behavior in Late Stages of Drosophila Oogenesis

Visualizing Lipid Droplets in Drosophila Oogenesis

Assessing Ovulation in Drosophila melanogaster

A Low-Tech Flow Chamber for Live Imaging of Drosophila Egg Chambers during Drug Treatments

Detection and Assessment of Wolbachia pipientis Infection

Measuring Transposable Element Activity in Adult Drosophila Ovaries

Preparation of Drosophila Ovarioles for Single-Cell RNA Sequencing

Chromatin Immunoprecipitation Experiments from Drosophila Ovaries

Detection of Actin in Nuclear Protein Fraction Isolated from Adult Drosophila Ovary

STAMP: Spatio-Temporal Association Mapping of Proteins

Using Drosophila Oogenesis in the Classroom to Increase Student Participation in Biomedical Research

Gaining Wings to FLY: Using Drosophila Oogenesis as an Entry Point for Citizen Scientists in Laboratory Research.

ISBN:

9781071629703

1071629700