Borrelia burgdorferi : Methods and Protocols / edited by Leona Gilbert.

Format:

Book

Contributor:

Gilbert, Leona., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2742

Language:

English

Subjects (All):

Bacteria.

Cytology.

Diseases.

Mechanisms of Disease.

Local Subjects:

Bacteria.

Mechanisms of Disease.

Physical Description:

1 online resource (XII, 280 p. 52 illus., 42 illus. in color.)

Edition:

1st ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This volume covers the latest advancements and techniques used to understand the fastidious bacterium, Borrelia burgdorferi, and its significance in infectious disorders by combining both conventional and cutting-edge approaches. This book covers diverse topics, including direct detection, diagnostic methods, immune response analysis, alternative model systems, advanced proteomics, social media analysis, and clinical research. It also discusses unconventional wet lab research such as content analysis, the use of ChatGPT, clinical algorithms for chronic Lyme, establishment of a pregnancy Lyme disease biobank, and investigates Lyme in pregnant women. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Borrelia burgdorferi: Methods and Protocols encompasses a wide range of techniques and caters to scientists from various disciplines and career stages, such as cell and molecular biologists, statisticians, and clinical researchers. .

Contents:

Real-Time Microfluidic PCRs: A High-Throughput Method to Detect 48 or 96 Tick-Borne Pathogens in 48 or 96 Samples

Direct Detection of Borrelia Species in Tissues

Purification of Borrelia burgdorferi Outer Membrane Vesicles

Constructing an ELISA for Detection of Anti-Borrelia in Wildlife and Agricultural Animals

A Step-by-Step Guide to Selecting an Optimal Cut-Off Value Based on the Receiver Operating Characteristic and Youden Index in Methods Designed to Diagnose Lyme Disease

Adaptive Immune Response Investigation in Lyme borreliosis

CYTOSPOT: Intracellular Staining and Detection of Cytokines by Flow Cytometer, An Alternative to LYMESPOT

Use of Specific Borrelia Phages as a New Strategy for Improved Diagnostic Tests

Identification of Unambiguous Borrelia Peptides in Human Urine Using Affinity Capture and Mass Spectrometry

Preparation of Borrelia Infected Mammalian Cells for Helium Ion Microscopy

Establishing a Zebrafish Model for Borrelia Burgdorferi Infection Using Immersion and Microinjection Methods

Development of Organoids to Study Infectious Host Interactions

Content Analysis: An Approach to Exploring the Depiction of Tick-Borne Diseases

Applying BERT and ChatGPT for Sentiment Analysis of Lyme Disease in Scientific Literature

Building a Binary Classification Machine Learning Model: A Guide to Predicting Participation in a Lyme Disease Program at a Medical Institute

Clinical Algorithm and Diagnostic Tools for Lyme Disease

Establishing a Pregnancy Lyme Disease Biobank

Developing a Prospective Gestational Lyme Disease Study.

ISBN:

9781071635612

1071635611