Microfluidics Diagnostics : Methods and Protocols / edited by Valérie Taly, Stéphanie Descroix, Karla Perez-Toralla.

Format:

Book

Contributor:

Taly, Valérie, Editor.

Descroix, Stéphanie, Editor.

Perez-Toralla, Karla, Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2804

Language:

English

Subjects (All):

Microfluidics.

Diagnosis.

Local Subjects:

Microfluidics.

Diagnosis.

Physical Description:

1 online resource (XII, 255 p. 74 illus., 70 illus. in color.)

Edition:

1st ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This detailed volume explores a wide range of microfluidic-based approaches that exploit the unique features of microfluidic devices, which hold significant potential in the field of diagnosis. Beginning with a section on microchips for sample preparation, including cell-free DNA, exosomes, and cells, the book continues by covering protein marker analysis and detection, single-cell analysis, analysis of bacteria and viruses, as well as human cell-based culture and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microfluidics Diagnostics: Methods and Protocols serves as an ideal guide for researchers investigating how microfluidics can shape the future of diagnosis.

Contents:

Self-Powered Microfluidics for Point-of-Care Solutions: From Sampling to Detection of Proteins and Nucleic Acids

DNA Sizing with pg/mL Sensitivity for Cell-Free DNA Analysis with the mLAS Technology

On-Chip Magnetic Extraction of Circulating Cell-Free DNA from Biological Samples

Isolation of Extracellular Vesicles from Cell Culture and Blood through Nano-Targeted DLD Microfluidic Device

Microchip for immunomagnetic sorting of Circulating Tumor Cells (CTCs)

Enhancing the Efficiency of Conventional Surface Immunoassays within Standard Labware Using Microscale Flows

Droplet Microfluidics with Capillary Electrophoresis for Glycan Biomarker Analysis

Microfluidic Cartridge for Bead-Based Affinity Assays

A Guide to the Quantitation of Protein Secretion Dynamics at the Single-Cell Level

Magnetic Microtweezers for High-Throughput Bioseparation in Sub-Nanoliter Droplets

A Thermoplastic Microsystem to Perform Antibiotic Susceptibility Testing by Monitoring Oxygen Consumption

An Immiscible-Phase Filtration Device for Isolation, Amplification, and Detection of Nucleic Acids for Clinical Diagnostics

Microfluidic 3D Cytotoxic Assay

Evaluating Nanoparticles Penetration by a New Microfluidic Hydrogel-Based Approach

Selective Targeting of Tumor Cells in a Microfluidic Tumor Model with Multiple Cell Types.

ISBN:

9781071638507