Recombinant Glycoproteins : Methods and Protocols / edited by Steven B. Bradfute.

Format:

Book

Contributor:

Bradfute, Steven B., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2762

Language:

English

Subjects (All):

Proteins.

Local Subjects:

Proteins.

Physical Description:

1 online resource (XIV, 355 p. 89 illus., 77 illus. in color.)

Edition:

1st ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This detailed volume explores the production and purification of glycoproteins for therapeutic use or basic research, as well as the necessary in-depth understanding of cellular and acellular systems available for these purposes, their advantages and disadvantages, and considerations for choosing the most appropriate system for the desired application. Beginning with chapters on viral-encoded glycoproteins and those found in other pathogens, the book continues by examining the production of mammalian glycoproteins, the analysis of glycoprotein content, cell-free synthesis of glycoproteins and considerations for production and purification of glycoproteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Glycoproteins: Methods and Protocols provides a wide range of guidelines for studying these vitally important proteins. Chapter “Solubilization of Oligomeric Cell-Free Synthesized Proteins Using SMA Copolymers” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Contents:

Production and Purification of Hantavirus Glycoproteins in Drosophila melanogaster S2 Cells

Production and Purification of Filovirus Glycoproteins

Modification of N-Linked Glycan Sites in Viral Glycoproteins

Production of Influenza Virus Glycoproteins Using Insect Cells

SARS-CoV-2 S-Protein – Ace2 Binding Analysis Using Surface Plasmon Resonance

A Biosensor Assay Based on Coiled-Coil-Mediated Human ACE2 Receptor Capture for the Analysis of Its Interactions with the SARS-CoV-2 Receptor Binding Domain

Production and Purification of Plasmodium Circumsporozoite Protein in Lactococcus lactis

Analysis of Caenorhabditis Protein Glycosylation

Use of Reductive Amination to Produce Capsular Polysaccharide-Based Glycoconjugates

Overexpression and Purification of Mitogenic and Metabolic Fibroblast Growth Factors

Production and Purification of Antibodies in Chinese Hamster Ovary Cells

Mammalian Antigen Display for Pandemic Countermeasures

Analysisof Native and Permethylated N-Glycan Isomers Using MGC-LC-MS Techniques

Targeted Glycoproteomics Analysis Using MRM/PRM Approaches

Targeted Analysis of Permethylated N-Glycans Using MRM/PRM Approaches

Hydrophilic Interaction Liquid Chromatography (HILIC) Enrichment of Glycopeptides Using PolyHYDROXYETHYL A

O-Glycoproteomics Sample Preparation and Analysis Using NanoHPLC and Tandem MS

Solubilization of Oligomeric Cell-Free Synthesized Proteins Using SMA Copolymers

Cell-Free Systems for the Production of Glycoproteins

Considerations for Glycoprotein Production.

ISBN:

9781071636664

1071636669