Cryptococcus neoformans : Methods and Protocols / edited by Erin E. McClelland.

Format:

Book

Contributor:

McClelland, Erin E., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2775

Language:

English

Subjects (All):

Microbiology.

Local Subjects:

Microbiology.

Physical Description:

1 online resource (XV, 425 p. 76 illus., 69 illus. in color.)

Edition:

1st ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This volume explores the latest developments in the study of Cryptococcus neoformans and its pathology, along with discussion on newly used therapeutics. The chapters in this book cover a wide range of protocols commonly used in Cryptococcus research such as animal models, genetics techniques, virulence factor phenotyping, and microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cryptococcus neoformans: Methods and Protocols is a valuable resource that will help researchers further enhance their understanding of this pathogenic yeast, and will aid in new discoveries and therapeutics. .

Contents:

A Galleria Model of Cryptococcus neoformans Infection

The Rabbit Model of Cryptococcal Meningitis

Models for Inducing Experimental Cryptococcosis in Mice

In Vivo Modeling of Cryptococcus neoformans Infection and Collection of Murine Samples

Biolistic Transformation of Cryptococcus neoformans

Genetic Transformation of Cryptococcus Species with Agrobacterium Transfer DNA

Gene Silencing via RNA Interference in Cryptococcus

Cryptococcus neoformans Transcriptome Analysis to Identify Differentially Expressed Genes Using the STAR Pipeline in CyVerse Discovery Environment

Proteomic Profiling of Samples Derived from a Murine Model Following Cryptococcus neoformans Infection

Electron Microscopy of Cryptococcus neoformans: Processing Challenges to Avoid Artifacts Glauber R. de S. Araújo, Bruno Pontes, and Susana Frases

Assessing Phagocytosis of Cryptococcus neoformans Cells in Human Monocytes or the J774 Murine Macrophage Cell Line

Interaction Between Macrophages and Cryptococcus neoformans: Distinguishing Phagocytosed Versus External Fungi

Detection and Quantification of Cryptococcus Uptake by Phagocytic Cells Using Imaging Flow Cytometry

Two Methods of Measuring Cryptococcus neoformans Fungal Burden in Macrophages

Quantification of C. neoformans Capsule Diameter

Methods of Cryptococcal Polysaccharide Analysis Using ELISA

Measuring Laccase Activity and Melanin Production in Cryptococcus neoformans

Measuring Urease and Phospholipase Secretion in Cryptococcus neoformans

Measuring Stress Phenotypes in Cryptococcus neoformans

Antibody Isolation in C. neoformans

Fluorescence and Biochemical Assessment of the Chitin and Chitosan Content of Cryptococcus

Induction of Dormancy in Cryptococcus neoformans In Vitro: The HypNOS Protocol

Analysis of Cryptococcus Extracellular Vesicles

Preparation of Biologically Active Fractions Enriched with Glucuronoxylomannan, the Main Antigen of the Cryptococcal Capsule

Measuring Replicative Lifespan in Cryptococcus neoformans

In Vitro Titan Cell Generation in Cryptococcus neoformans and Automated Cell Size Measurements

Chitosan-Deficient Cryptococcus as Whole-Cell Vaccines

Vaccine Strategies for Cryptococcus neoformans.

ISBN:

9781071637227

1071637223

OCLC:

1460466603