Patient-Derived Xenografts : Methods and Protocols / edited by Mohamed I. Saad.

Format:

Book

Contributor:

Saad, Mohamed I., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2806

Language:

English

Subjects (All):

Cancer.

Cancer--Animal models.

Medicine--Research.

Medicine.

Biology--Research.

Biology.

Cancer Biology.

Cancer Models.

Translational Research.

Local Subjects:

Cancer Biology.

Cancer Models.

Translational Research.

Physical Description:

1 online resource (XII, 244 p. 49 illus., 44 illus. in color.)

Edition:

1st ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This detailed volume explores the dynamic landscape of patient-derived xenograft (PDX) generation and applications. After a few chapters examining the evolution of PDXs, tracing their roots and contextualizing their significance in the broader narrative of cancer research, the book continues with methodologies for generating PDXs from diverse clinical tissue samples as well as for utilizing PDXs in various specific cancer types. From the development of humanized PDX models to the exploration of protein expression through advanced techniques including multiplex immunohistochemistry, Western blotting, and immunoprecipitation, the chapters offer a comprehensive toolkit for researchers and clinicians alike. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patient-Derived Xenografts: Methods and Protocols serves as an ideal interdisciplinary guide for understanding the intricate processes that underscore translational cancer research.

Contents:

Patient-Derived Xenografts: Historical Evolution, Immunocompromised Host Models, and Translational Significance

Patient-Derived Xenograft Models in Cancer Research: Methodology, Applications, and Future Prospects

Patient-Derived Xenografts: A Valuable Preclinical Model for Drug Development and Biomarker Discovery

Patient-Derived Xenograft Models for Leukemias

Patient-Derived Orthotopic Xenograft Models for High-Grade Pediatric Brain Cancers

Generation of Orthotopic and Subcutaneous Patient-Derived Xenograft Models from Diverse Clinical Tissue Samples of Pediatric Extracranial Solid Tumors

Development of Orthotopic Patient-Derived Xenograft Models of Pediatric Intracranial Tumors

A Humanized Patient-Derived Xenograft Model for Pancreatic Cancer

Development and Expansion of Patient-Derived Xenografts for Endometrial Cancer

A Multiplexed Approach to Assess Small Cell Lung Cancer Subtype Heterogeneity in Primary and Patient-Derived Tumor Samples

Generation of Metastatic Cholangiocarcinoma Patient-Derived Xenograft Models

Patient-Derived Xenograft Models for Translational Prostate Cancer Research and Drug Development

Patient-Derived Xenograft Models for Ovarian Cancer

Orthotopic MC-38 Allograft as a Robust Preclinical Model of Colorectal Carcinoma

Assessing Protein Expression in Patient-Derived Xenografts Using Western Blotting

Analysis of Protein Interactions in Patient-Derived Xenografts Using Immunoprecipitation

A Semi-Automated Proteomics and Phosphoproteomics Protocol for the Identification of Novel Therapeutic Targets and Predictive Biomarkers in In Vivo Xenograft Models of Pediatric Cancers.

ISBN:

9781071638583