Inflammation and Cancer : Methods and Protocols / edited by Brendan J. Jenkins.

Format:

Book

Contributor:

Jenkins, Brendan J., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2691

Language:

English

Subjects (All):

Cancer.

Cancer Biology.

Local Subjects:

Cancer Biology.

Physical Description:

1 online resource (XVI, 373 p. 72 illus., 61 illus. in color.)

Edition:

2nd ed. 2023.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

Summary:

This second edition volume expands on the previous edition with an update on the broad spectrum of research models, techniques, and protocols used in laboratories by basic and clinical researchers. The chapters in this book are divided into two parts. Part One discusses the latest findings on the development and characterization of representative research models for chronic immune-based diseases and inflammation-associated cancers. Part Two covers biochemical, molecular, and cellular biological techniques that are commonly used to dissect the molecular mechanisms and cellular processes that drive the pathogenesis of certain disease states. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Inflammation and Cancer: Methods and Protocols, Second Edition is a valuable resource for those with a diverse range of laboratory-based experience, ranging from novice undergraduate students to established basic or clinical researchers who wish to diversify their existing portfolio of practical knowledge in the field. .

Contents:

Identifying Adult Stomach Tissue Stem/Progenitor Cells using the Iqgap3-2A-CreERT2 Mouse

In Vitro and In Vivo Models for Metastatic Intestinal Tumors using Genotype-Defined Organoids

On Target: An Intrapulmonary Transplantation Method for Modelling Lung Tumor Development in Its Native Microenvironment

Endoscopic Ultrasound-Guided Fine Needle Biopsies to Generate Preclinical Disease Models to Study Inflammation in Pancreatic Ductal Adenocarcinoma

Modeling Intestinal Carcinogenesis using In Vitro Organoid Cultures

An In Vitro Model for Assessing Acute Lung Injury during Pancreatitis Development using Primary Mouse Cell Co-Cultures

Tracking the Host Response to Infection in Peritoneal Models of Acute Resolving Inflammation

Assessing Lung Inflammation and Pathology in Preclinical Models of Chronic Obstructive Pulmonary Disease

Preclinical Mouse Model of Silicosis

Tracking Cardiovascular Comorbidity in Models of Chronic Inflammatory Disease

Live Imaging of Pyroptosis in Primary Murine Macrophages

A Streamlined Method for Detecting Inflammasome-Induced ASC Oligomerization using Chemical Crosslinking

DNA Methylation Analysis

Flow Cytometry Identification of Cell Compartments in the Murine Brain

Expression and Purification of Inflammasome Sensor Nod-Like Receptor Protein-1 using the Baculovirus-Insect Cell Expression System

Dissecting lnterleukin-6 Classic- and Trans-Signaling in Inflammation and Cancer

Selecting Therapeutic Antisense Oligonucleotides with Gene Targeting and TLR8 Potentiating Bifunctionality

Exploring Allosteric Inhibitors of Protein Tyrosine Phosphatases through High-Throughput Screening

Intravital Imaging of Regulatory T Cells in Inflamed Skin

Confocal Endomicroscopy Monitoring of Tumor Formation

Detection of Free Bioactive IL-18 and IL-18BP in Inflammatory Disorders

MAC-seq: Coupling Low-Cost, High-Throughput RNA-seq with Image-Based Phenotypic Screening in 2D and 3D Cell Models

Primary Intestinal Fibroblasts: Isolation, Cultivation, and Maintenance

Lipid Nanoparticle-Mediated Delivery of miRNA Mimics to Myeloid Cells

Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation.

ISBN:

9781071633311

1071633317