RNA Vaccines : Methods and Protocols / edited by Thomas Kramps.

Format:

Book

Contributor:

Kramps, Thomas, Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2786

Language:

English

Subjects (All):

Biotechnology.

Immunology.

Virology.

Genetics.

Local Subjects:

Biotechnology.

Immunology.

Virology.

Genetics.

Physical Description:

1 online resource (XII, 389 p. 57 illus., 49 illus. in color.)

Edition:

2nd ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This second edition volume expands on the previous edition with discussions of the latest technology and advancements in the development of RNA vaccines and proposed solutions on how to tackle currently unmet medical needs. The chapters in this book are organized into four parts and cover topics such as replication and non-replicating RNA vectors, formulation and delivery of RNA vaccines, and clinical and nonclinical development of RNA vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensive, RNA Vaccines: Methods and Protocols, Second Edition is a valuable resource for any researcher interested in learning more about this important and developing field. .

Contents:

Introduction to RNA Vaccines Post COVID-19

Self-Replicating RNA Derived from the Genomes of Positive Strand RNA Viruses

Negative Strand RNA Virus Vectored Vaccines

New Generation Self-Replicating RNA Vaccines Derived from Pestivirus Genome

Trans-Amplifying RNA Vaccines Against Infectious Diseases: A Comparison with Non-Replicating and Self-Amplifying RNA

Generation and Characterization of In Vitro Transcribed mRNA

Production and Evaluation of Nucleoside-Modified mRNA Vaccines for Infectious Diseases

Sequence-Optimized mRNA Vaccines Against Infectious Disease

Design and Synthesis of Circular RNA Expression Vectors

Electroporation of mRNA as a Universal Technology Platform to Transfect a Variety of Primary Cells with Antigens and Functional Proteins

A Basic Method for Formulating mRNA-Lipid Nanoparticle Vaccines in the Lab

Messenger RNA Lipid-Based Nanoparticles: Optimization of Formulations in the Lab

Plant Expression of Trans-Encapsidated Chimeric Viral Vaccines with Animal RNA Replicons: An Update

Producing Plasmid DNA Template for Clinical Grade RNA Vaccine Manufacture

Regulatory Considerations for Producing mRNA Vaccines for Clinical Trials

Quality by Digital Design for Developing Platform RNA Vaccine Manufacturing Processes

Nonclinical Safety Testing of RNA Vaccines.

ISBN:

9781071637708