Xylem : Methods and Protocols / edited by Javier Agusti.

Format:

Book

Contributor:

Agusti, Javier., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2722

Language:

English

Subjects (All):

Botany.

Plant Science.

Local Subjects:

Plant Science.

Physical Description:

1 online resource (XI, 245 p. 50 illus., 45 illus. in color.)

Edition:

2nd ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This second edition provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue. Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization though imaging techniques, and analysis of the xylem composition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Xylem: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .

Contents:

Monitoring Xylem Transport in Arabidopsis thaliana Seedlings Using Fluorescent Dyes

Modeling and analyzing xylem vulnerability to embolism as an epidemic process

Modeling Xylem Functionality Aspects

Detecting and quantifying xylem embolism by synchrotron-based x-ray micro-CT

Analysis of xylem cells by nucleus-based transcriptomics and chromatin profiling

Quantification of xylem-specific thermospermine-dependent translation of SACL transcripts with dual luciferase reporter system

Fossil wood analyses: several examples from five case studies in the area of Central and NW Bohemia, Czech Republic

Isolation and reproductive structures induction of fungal pathogens associated with xylem and wood necrosis in grapevine

Determination of de novo suberin-lignin ferulate deposition in xylem tissue upon vascular pathogen attack

Quantification of treacheary elements types in mature hypocotyl of Arabidopsis thaliana

Histochemical Detection of Peroxidase and Laccase Activities in Populus Secondary Xylem

Lignin analysis by HPLC and FTIR: spectra deconvolution and S/G ratio determination

Inducible pluripotent suspension cell cultures (iPSCs) to study plant cell differentiation

Bulk and in situ quantification of coniferaldehyde residues in lignin

Clearing of vascular tissue in Arabidopsis thaliana for reporter analysis of gene expression.

ISBN:

9781071634776

1071634771